caa a22 4500 469030631 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF01460826 doi (NATIONALLICENCE)springer-10.1007/BF01460826 Electron microscopic immunocytochemical localization of proline-rich proteins in normal mouse parotid salivary glands [Elektronische Daten] [H. Mansouri, G. Cope, N. Divecha, C. Mcdonald] Summary: Rabbit polyclonal antibodies against isoproterenol-induced mouse proline-rich proteins (PRPs) were used to localize PRPs in the parotid salivary glands of normal adult BALB/c mice. The antibodies recognized both acidic-type and basic-type PRPs. Immunoblotting experiments revealed that the glands contained an acidic-type and a basic-type PRP. Parotid gland tissue was fixed with Karnosky's fixative and embedded in Lowicryl resin at low temperature. PRPs were localized at the electron microscope level using an indirect post-embedding staining technique with protein A-gold. The secretion granules of the acinar cells were strongly labelled. Pre-absorption of the antibody with purified acidic-type and basic-type PRPs indicated that the basic-type PRP is mainly located at the periphery of the granules but that the acidic-type PRP is more evenly distributed within the granules. Pre-absorption of the antibody with α-amylase did not affect the staining pattern, suggesting minimal cross-reactivity. PRPs were also detected within the rough endoplasmic reticulum and the Golgi apparatus of acinar cells, within the granules of the proacinar cells and in the lumena of the ducts, but not within the intercalated or striated duct cell granules. Chapman & Hall, 1992 Mansouri H. Department of Biomedical Science, University of Sheffield, S10 2TN, Sheffield, UK aut Cope G. Department of Biomedical Science, University of Sheffield, S10 2TN, Sheffield, UK aut Divecha N. The Krebs Institute for Biomolecular REsearch, Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut Mcdonald C. The Krebs Institute for Biomolecular REsearch, Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 737-746 0018-2214 24:10<737 1992 24 10735 https://doi.org/10.1007/BF01460826 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01460826 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mansouri H. Department of Biomedical Science, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 700 1- Cope G. Department of Biomedical Science, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 700 1- Divecha N. The Krebs Institute for Biomolecular REsearch, Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 700 1- Mcdonald C. The Krebs Institute for Biomolecular REsearch, Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 737-746 0018-2214 24:10<737 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer