caa a22 4500 469030666 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF01460824 doi (NATIONALLICENCE)springer-10.1007/BF01460824 Localization of laminin in nephritic glomeruli as revealed by a quick-freezing and deep-etching method with immunohistochemistry [Elektronische Daten] [Atsuhiko Naramoto, Shinichi Ohno, Nobuo Itoh, Nobuo Shibata, Hidekazu Shigematsu] Summary: The three-dimensional localization of laminin in rat glomeruli at the chronic phase of Masugi nephritis was investigated by a quick-freezing and deep-etching method combined with immunohistochemistry. Light-microscopically, laminin was localized in increased mesangial matrix and thickened glomerular basement membrane. The quick-freezing and deep-etching method revealed that the increased mesangial matrix, which was newly formed in axial portions and areas of mesangial interposition, was composed of fine fibrillar networks. They were revealed with the 3,3′-diaminobenzidine tetrahydrochloride (DAB) reaction products of peroxidase-labelled secondary antibody following anti-laminin antibody. However, these reaction products were not uniformly distributed in the newly formed matrix. Although the fibrils organizing lamina densa were also immunostained with anti-laminin antibody, the fibrils connected to mesangial cells, podocytes and endothelial cells had smaller amounts of DAB reaction products for laminin. These results indicate that one of the components of fibrils in the mesangial matrix and lamina densa is laminin, which is heterogeneously distributed in the newly formed matrix. Chapman & Hall, 1992 Naramoto Atsuhiko Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Ohno Shinichi Department of Anatomy, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Itoh Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Shibata Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Shigematsu Hidekazu Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 717-726 0018-2214 24:10<717 1992 24 10735 https://doi.org/10.1007/BF01460824 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01460824 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Naramoto Atsuhiko Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Ohno Shinichi Department of Anatomy, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Itoh Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Shibata Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Shigematsu Hidekazu Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 717-726 0018-2214 24:10<717 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer