caa a22 4500 469030682 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF01460827 doi (NATIONALLICENCE)springer-10.1007/BF01460827 Immunoelectron microscopic studies of glycosaminoglycans in the metaphyseal bone trabeculae of growing rats [Elektronische Daten] [Toshitada Kazama, Minoru Takagi, Teruhiko Ishii, Yoshihisa Toda] Summary: The types and distribution of glycosaminoglycans (GAGs) were studied immunocytochemically in osteoid, mineralized bone matrix, and cartilage matrix of growing rat metaphyseal bone after aldehyde fixation and EDTA demineralization, using four monoclonal antibodies (mAbs 1-B-5, 2-B-6, 3-B-3 and 5-D-4). These mAbs specifically recognize epitopes in non-sulphated chondroitin (C0-S); chondroitin 4-sulphate (C4-S) and dermatan sulphate (DS); chondroitin 6-sulphate (C6-S) and C0-S; and keratan sulphate (KS) respectively. In osteoid, all mAbs except 1-B-5 weakly stained matrix material on and between collagen fibrils, and moderately stained organic material corresponding to bone nodules, which are known sites of mineralization. However, the staining of osteoid abruptly decreased at the mineralization front; weak staining was confined mostly to the organic material of bone nodules in mineralized bone matrix, with very weak or no staining of the rest of the bone matrix. This staining progressively decreased toward the mineralized cartilage matrix and became negative. The mineralized cartilage matrix and lamina limitans reacted strongly with all mAbs except 5-D-4. These results indicate that osteoid contains sulphated proteoglycans containing C4-S and/or DS, C6-S and KS, and subsequent bone matrix mineralization appears to require accumulation of these macromolecules within bone nodules and eventual loss of these substances for complete mineralization, whereas proteoglycans containing C0-S, C4-S and/or DS, and C6-S, still exist in mineralized cartilage matrix and lamina limitants. Chapman & Hall, 1992 Kazama Toshitada Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut Takagi Minoru Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut Ishii Teruhiko Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut Toda Yoshihisa Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 747-755 0018-2214 24:10<747 1992 24 10735 https://doi.org/10.1007/BF01460827 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01460827 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kazama Toshitada Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Takagi Minoru Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Ishii Teruhiko Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Toda Yoshihisa Department of Oral Surgery (TK, TI) and Anatomy (MT, YT), Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, 101, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/10(1992-10-01), 747-755 0018-2214 24:10<747 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer