caa a22 4500 469030798 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF01046789 doi (NATIONALLICENCE)springer-10.1007/BF01046789 Stirling John Department of Pathology, Flinders Medical Centre, 5042, Bedford Park, South Australia, Australia aut Unfixed tissue for electron immunocytochemistry: a simple preparation method for colloidal gold localization of sensitive epitopes using ethanediol dehydration [Elektronische Daten] [John Stirling] Summary: A quick, simple protocol is described for the preparation of tissue for electron immunocytochemistry without the use of fixatives or deleterious solvents. Fresh, normal human colon was rapidly dehydrated in ethanediol (ethylene glycol) then embedded directly in low-acid glycol methacrylate. Using both mono-and polyclonal antibodies, in conjunction with colloidal gold probes, a range of intra-and extracellular epitopes were localized; these epitopes included lysozyme, chromogranin, desmin and collagen IV. Overall, the tissue compared well with material fixed in glutaraldehyde, partially dehydrated and embedded in LR White acrylic resin. Ultrastructural detail was good and was further enhanced, without affecting probe density and epitope localization, by the addition of 1% tannic acid or 1% uranyl acetate to the dehydrant. The technique is applicable to a wide range of tissues, allowing excellent antigen retention which might prove useful for the immunolocalization of sensitive epitopes. Chapman & Hall, 1992 The Histochemical Journal Kluwer Academic Publishers 24/4(1992-04-01), 190-206 0018-2214 24:4<190 1992 24 10735 https://doi.org/10.1007/BF01046789 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046789 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Stirling John Department of Pathology, Flinders Medical Centre, 5042, Bedford Park, South Australia, Australia aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/4(1992-04-01), 190-206 0018-2214 24:4<190 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer