caa a22 4500 469030828 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01046842 doi (NATIONALLICENCE)springer-10.1007/BF01046842 Kawamata S. Department of Anatomy, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan aut The distribution of calcium in undecalcified bone as revealed by an improved pyro-antimonate method [Elektronische Daten] [S. Kawamata] Summary: The localization of pyro-antimonate-precipitable Ca2+ in the undecalcified femur and calvaria of neonatal rats was examined. The fixation of bones with pyro-antimonate-glutaraldehyde followed by pyro-antimonate-osmium, (two-step method) resulted in better preservation of tissue and more precise localization of precipitates than did the direct immersion of specimens in pyro-antimonate-osmium solution (one-step method). The precipitate was frequently observed within the endoplasmic reticulum of obsteoblasts. Most vacuoles in osteoclasts contained precipitate. By contrast, the mitochondria in these cells were associated with small amounts of precipitate. There was no evidence of precipitate in the Golgi apparatus. The presence of calcium in the precipitate was verified by EGTA treatment and X-ray microanalysis. This study demonstrated that (1) the two-step pyro-antimonate method is a useful and reliable procedure for visualizing Ca2+, and (2) cellular Ca2− can be successfully localized in undecalcified bone by this method. Chapman & Hall, 1992 The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 275-282 0018-2214 24:5<275 1992 24 10735 https://doi.org/10.1007/BF01046842 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046842 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kawamata S. Department of Anatomy, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 275-282 0018-2214 24:5<275 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer