caa a22 4500 469030852 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01046840 doi (NATIONALLICENCE)springer-10.1007/BF01046840 Presence of cardiac α-myosin correlates with histochemical myosin Ca2+ ATPase activity in rabbit masseter muscle [Elektronische Daten] [J. Bredman, W. Weijs, A. Moorman] Summary: A combined enzyme-histochemical (ATPase reactivity) and immunohistochemical study has been performed on sections of rabbit masseter muscle. The majority of the fibres previously designated as type IIC and/or type I according to their ATPase activity were found to contain ‘cardiac' α-myosin heavy chain in addition to other myosin heavy chains. All α-myosin heavy chain-containing fibres reveal ATPase activity after pre-incubation at pH 4.2-4.6 similar to that of the classical type I fibres, while in that pH range, limb type IIC fibres show intermediate ATPase activity. One group of these fibres reveal ATPase activity after pre-incubation at pH 10.1-10.3 as well, but not at pH 10.4-10.5. These fibres contain exclusively either α- or α- and I-myosin heavy chains but do not contain the IIA-myosin heavy chain. The second part of the fibres reveals ATPase activity after treatment within the whole alkaline pre-incubation range (pH 10.1-10.5) and these fibres contain α-myosin and IIA-myosin but no, I-myosin heavy chain. It is concluded that the classical IIC fibre type is not present in the rabbit masseter muscle. Furthermore, ATPase reactivity does not allow us to distinguish fibres on their myosin heavy chain content in rabbit masseter muscle. Chapman & Hall, 1992 Bredman J. Department of Functional Anatomy, Academic Centre for Dentistry, Amsterdam (ACTA), The Netherlands aut Weijs W. Department of Functional Anatomy, Academic Centre for Dentistry, Amsterdam (ACTA), The Netherlands aut Moorman A. Department of Anatomy and Embryology, Faculty of Medicine, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands aut The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 260-265 0018-2214 24:5<260 1992 24 10735 https://doi.org/10.1007/BF01046840 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046840 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bredman J. Department of Functional Anatomy, Academic Centre for Dentistry, Amsterdam (ACTA), The Netherlands aut NATIONALLICENCE 700 1- Weijs W. Department of Functional Anatomy, Academic Centre for Dentistry, Amsterdam (ACTA), The Netherlands aut NATIONALLICENCE 700 1- Moorman A. Department of Anatomy and Embryology, Faculty of Medicine, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 260-265 0018-2214 24:5<260 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer