caa a22 4500 469030879 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01046843 doi (NATIONALLICENCE)springer-10.1007/BF01046843 Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells [Elektronische Daten] [Olle Johansson, Markku Virtanen, Marita Hilliges, Qiner Yang] Summary: Mast cells have characteristic granulae containing various glucoseaminoglycans, proteases and amines (predominantly histamine). The conventional histological methods for studying mast cells are based upon acidic ortho-and metachromatic routine stains of the glucoseaminoglycans. However, the success of these procedures is dependent upon both the fixatives and the tissues used. In this study, we wanted to find out whether an immunohistochemical procedure could overcome some of these difficulties. Normal human skin was fixed in five different types of fixative and processed for indirect immunofluorescence, using an antiserum to histamine. Only one, 4% carbodiimide in 0.1m phosphate buffer (pH 7.4), resulted in immunostaining. The quality of the staining was good, with a high signal-to-noise ratio, and was located on the mast cells. The method, made it possible to visualize small structures such as a single secreted granula, the thin cytoplasmatic extension of some cells, and a previously undescribed dendritic morphology of some of the mast cells. We therefore recommend, this procedure for cellular studies of mast cells when accuracy is needed. Chapman and Hall, 1992 Johansson Olle Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut Virtanen Markku Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut Hilliges Marita Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut Yang Qiner Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 283-287 0018-2214 24:5<283 1992 24 10735 https://doi.org/10.1007/BF01046843 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046843 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johansson Olle Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut NATIONALLICENCE 700 1- Virtanen Markku Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut NATIONALLICENCE 700 1- Hilliges Marita Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut NATIONALLICENCE 700 1- Yang Qiner Experimental Dermatology Unit, Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 283-287 0018-2214 24:5<283 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer