caa a22 4500 469030887 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01046841 doi (NATIONALLICENCE)springer-10.1007/BF01046841 Distribution of taurine in the rat cerebellum and insect brain: application of a new antiserum against carbodiimide-conjugated taurine [Elektronische Daten] [Ulla Pirvola, Pertti Panula] Summary: The production, specificity and application of an antiserum against taurine conjugated to succinylated ovalbumin by means of 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide is reported. The antiserum was produced in rabbits. The carbodiimide was used also as a tissue fixative. The development of the antibody titre was followed with dot-blot tests on nitrocellulose filters using different amino acid conjugates and with immunohistochemical reaction in the rat and insect brain. Blocking controls were also used. Taurine antiserum, sufficiently specific and sensitive, developed after the fourth booster injection, after which the antiserum was characterized. In the insect brain, intense taurine-like immunoreactivity was observed in the photoreceptors, in the Kenyon cells and the neuropile of the mushroom bodies, in the lower part of the central body and in the antennal lobes. In the rat carebellum, intense taurine-like immunoreactivity was seen in the Purkinje cells. Immunoreaction was seen also in small cells most probably corresponding to the basket cells. The use of the carbodiimide in the production of antisera against taurine provides a parallel method for comparison of the distribution of taurine-like immunoreactivity obtained with antisera made against conjugates prepared with aldehydes. Chapman & Hall, 1992 Pirvola Ulla Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170, Helsinki, Finland aut Panula Pertti Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170, Helsinki, Finland aut The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 266-274 0018-2214 24:5<266 1992 24 10735 https://doi.org/10.1007/BF01046841 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046841 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pirvola Ulla Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170, Helsinki, Finland aut NATIONALLICENCE 700 1- Panula Pertti Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170, Helsinki, Finland aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/5(1992-05-01), 266-274 0018-2214 24:5<266 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer