caa a22 4500 469030909 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082441 doi (NATIONALLICENCE)springer-10.1007/BF01082441 Immunolocalization of proliferating perisinusoidal cells in rat liver [Elektronische Daten] [S. Johnson, J. Hines, A. Burt] Summary: There is now substantial evidence that perisinusoidal (Ito or fat-storing) cells are the principal source of extracellular matrix proteins during hepatic fibrogenesis. In rat liver these cells express the intermediate fiament protein desmin; this is now widely used as an immunohistochemical marker for these cells. It has been shown that in experimental models of acute and chronic liver injury there is an increase in the number of desmin-positive perisinusoidal cells prior to the deposition of matrix proteins; however, these studies have failed to establish whether local proliferation is involved in this expansion of the desmin-positive perisinusoidal cell population. In order to investigate the kinetics of the perisinusoidal cell response, we have developed a novel double-labelling immunohistochemical technique for the simultaneous demonstration of desmin and incorporated bromodeoxyuridine in proliferating perisinusoidal cells in sections of fixed paraffiin-embedded rat liver. Application of this technique to a model of acute liver injury (single dose carbon tetrachloride by gavage) has shown that expansion of the perisinusoidal cell population is contributed to by local proliferation, with a labelling index of 18.7% 2 days following injury. Chapman & Hall, 1992 Johnson S. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut Hines J. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut Burt A. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 67-72 0018-2214 24:2<67 1992 24 10735 https://doi.org/10.1007/BF01082441 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082441 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson S. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Hines J. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Burt A. Division of Pathology, School of Pathological Sciences, University of Newcastle upon Tyne, Royal Victoria Infirmary, NE1 4LP, Newcastle upon Tyne, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 67-72 0018-2214 24:2<67 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer