caa a22 4500 469030917 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082440 doi (NATIONALLICENCE)springer-10.1007/BF01082440 Fujimori O. Department of Anatomy, Nagoya City University Medical School, 467, Nagoya, Japan aut A double protein A-gold-silver staining method for tissue antigens in light microscopy [Elektronische Daten] [O. Fujimori] Summary: A double staining method has been developed for light microscopic immunohistochemistry. The technique consists of a sequence of protein A-gold-silver procedures, in which the reaction products are visualized in black and brown colours. The results obtained in the rat pancreatic islets and adenohyophysis, in combination with a variety of histochemical controls, substantiate both the specificity and reliability of this method. The double staining method is simple and economical, since the two differently coloured reaction products are obtained by a common mechanism of colouration (physical development) using a single probe, protein A-gold. Chapman & Hall, 1992 The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 61-66 0018-2214 24:2<61 1992 24 10735 https://doi.org/10.1007/BF01082440 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082440 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Fujimori O. Department of Anatomy, Nagoya City University Medical School, 467, Nagoya, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 61-66 0018-2214 24:2<61 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer