caa a22 4500 469030925 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082442 doi (NATIONALLICENCE)springer-10.1007/BF01082442 Reduction of the rate of fluorescence decay of FITC- and carboxyfluorescein-stained cells by anti-FITC antibodies [Elektronische Daten] [Ramadan Abuknesha, Hani Al-Mazeedi, Robert Price] Summary: Anti-fluorescein antibodies were found to prevent the fading of emitted fluorescence from fibroblasts stained with fluorescein-labelled fibronectin antibodies. The prevention of fading is the result of specific binding of the fluorochromes present on the stained cells by the anti-fluorescein antibodies. The sheep anti-FITC antibody used in this study was equally effective in preventing the fading of both FITC-and carboxyfluorescein-labelled fibronectin antibodies. The method is simple, effective, does not interfere with the primary immune reaction, and in addition to preventing the fading of fluorescence it reduced the background fluorescence of the specimens. The procedure is expected to make an important contribution to improving the quality of fluorescence immunohistochemical techniques used in diagnosis. Chapman & Hall, 1992 Abuknesha Ramadan Biochemistry and Biosensors Group, Chemistry Department, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut Al-Mazeedi Hani Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut Price Robert Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 73-77 0018-2214 24:2<73 1992 24 10735 https://doi.org/10.1007/BF01082442 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082442 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abuknesha Ramadan Biochemistry and Biosensors Group, Chemistry Department, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 700 1- Al-Mazeedi Hani Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 700 1- Price Robert Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 73-77 0018-2214 24:2<73 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer