caa a22 4500 46903095X CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082448 doi (NATIONALLICENCE)springer-10.1007/BF01082448 A novel technique for immunohistoperoxidase staining of unfixed whole joints of small animals [Elektronische Daten] [S. Meacock, D. Brandon, C. Brown, B. Swann] Summary: A method has been developed to cut unfixed and undecalcified sections of rat paws from animals with adjuvant arthritis and to stain them by a biotin-avidin immunoperoxidase technique. Good tissue integrity and morphology throughout the immunohistochemical procedure were retained if the sections were first mounted on transparent sellotape. The method is illustrated with two monoclonal antibodies (mAb) and is generally applicable with any mAb or polyclonal antibody and with joints from other small animals. For rats with adjuvant arthritis it was found important to block endogenous peroxidase before immunostaining. Complete inhibition of this enzyme without loss of antigenicity was best achieved after application of the mAb and biotinylated anti-IgG conjugate to the unfixed tissue sections. Chapman & Hall, 1992 Meacock S. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut Brandon D. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut Brown C. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut Swann B. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 115-119 0018-2214 24:2<115 1992 24 10735 https://doi.org/10.1007/BF01082448 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082448 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meacock S. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut NATIONALLICENCE 700 1- Brandon D. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut NATIONALLICENCE 700 1- Brown C. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut NATIONALLICENCE 700 1- Swann B. Lilly Research Centre Limited, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 115-119 0018-2214 24:2<115 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer