caa a22 4500 469030968 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082446 doi (NATIONALLICENCE)springer-10.1007/BF01082446 Immunofluorescent localization of pig complement component 3, regardless of the presence or absence of detectable immunoglobulins, in hyperacutely rejected heart xenografts [Elektronische Daten] [M. Wang, P. Johnston, L. Wright, S. Lim, D. White] Summary: Rabbit heart xenografts transplanted into the neck of newborn pigs were all hyperacutely rejected within two hours regardless of the presence or absence of detectable endogenous immunoglobulins (Ig). Cryostat tissue sections were prepared from the rejected rabbit hearts and incubated with sheep polyclonal antibodies against pig complement component 3 (C3), pig IgG and pig IgM. Specific immunoreaction was visualized by fluorescein-conjugated antibodies to sheep IgG. C3 was localized mainly on the surfaces of vascular endothelial as well as myocardial cells, and the localization was not dependent upon the presence of pig immunoglobulins within the same tissue. Both pig IgG and IgM were detected only in the heart xenografts transplanted into suckled pigs, whereas no trace of immunoglobulin was found in those transplanted into circulating antibody-free presuckled pigs. Treatment with cobra venom factor (which inhibits complement activity) prior to transplantation prolonged xenograft survival and completely abolished C3 immunostaining. The results provide new evidence at the histochemical level that the alternative pathway of complement is involved in hyperacute xenograft rejection of the species combination (rabbit to pig) used in this study. Chapman & Hall, 1992 Wang M. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut Johnston P. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut Wright L. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut Lim S. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut White D. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 102-109 0018-2214 24:2<102 1992 24 10735 https://doi.org/10.1007/BF01082446 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082446 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang M. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut NATIONALLICENCE 700 1- Johnston P. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut NATIONALLICENCE 700 1- Wright L. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut NATIONALLICENCE 700 1- Lim S. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut NATIONALLICENCE 700 1- White D. Department of Surgery, University of Cambridge Clinical School, Level 9, Addenbrooke's Hospital, Hills Road, CB2 2QQ, Cambridge, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 102-109 0018-2214 24:2<102 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer