caa a22 4500 469030976 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01082444 doi (NATIONALLICENCE)springer-10.1007/BF01082444 Alternating laminated array of two types of mucin in the human gastric surface mucous layer [Elektronische Daten] [Hiroyoshi Ota, Tsutomu Katsuyama] Summary: Attempts have been made to develop a procedure for preserving and analysing the surface mucous layer of the human stomach in paraffin sections. Histologically normal gastric mucosae were obtained from 20 surgically removed stomachs. Of the different fixatives tested, Carnoy's solution gave rise to the most satisfactory results. In Haematoxylin-Eosin stained sections, the surface mucous layer appeared as a thick eosinophilic layer coating the gastric mucosal surface and measured 55.4±2.5 μm in the fundus and 21.8±1.0 μm in the pylorus respectively. A dual staining method consisting of galactose oxidase-cold thionine Schiff and paradoxical concanavalin A staining was applied to the surface mucous layer in order to reveal the distribution pattern of mucins secreted by two types of mucous cell in the gastric mucosa: surface mucous cells and gland mucous cells. As a result of this staining, an alternating laminated layer was visualized which consisted of the particular two types of mucin. In five cases, the surface mucous layer was examined in unfixed frozen sections. This layer was only partially preserved but revealed the same laminated structure. These results indicated that gland mucous cell mucins contribute to form the surface mucous layer. Chapman & Hall, 1992 Ota Hiroyoshi Department of Laboratory Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut Katsuyama Tsutomu Department of Laboratory Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 86-92 0018-2214 24:2<86 1992 24 10735 https://doi.org/10.1007/BF01082444 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01082444 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ota Hiroyoshi Department of Laboratory Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Katsuyama Tsutomu Department of Laboratory Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/2(1992-02-01), 86-92 0018-2214 24:2<86 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer