caa a22 4500 469031085 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01046358 doi (NATIONALLICENCE)springer-10.1007/BF01046358 Transient expression of stage-specific embryonic antigen-1 (CD15) in the developing dorsal rat spinal cord [Elektronische Daten] [Martin Oudega, Enrico Marani, Rath Thomeer] Summary: The localization of CD15 (synonyms: stage-specific embryonic antigen-1 (SSEA-1), 3(α)-fucosyl-N-acetyl-lactosamine or FAL), which is implicated in neuronal differentiation, in the developing dorsal rat spinal cord was studied by immunocytochemistry. A embryonal day 9 (E9), SSEA-1 was detected in the neural ectoderm and, at E11, in cells near the ventricle of the matrix layer. This localization indicated that SSEA-1 is present in proliferating premigratory cells of the rat spinal cord. Between E12 and E16, cells of the alar plate expressed SSEA-1. Expression of the antigen was restricted to neuroblasts that will form the dorsal horn. SSEA-1, therefore, can be used at this stage as a marker for a subdivision of the matrix layer. At E14, the dorsal root entrance zone showed SSEA-1. This indicated that SSEA-1 is associated with ingrowing primary afferents. From E16 on, SSEA-1 was present in the dorsal raphe, which suggested a function for SSEA-1 in the guidance of developing fibres. After E17, the antigen was also found within the dorsal mantle layer. SSEA-1 was first present in Rexed's laminae II, IV and V. Later on in development the antigen was detected only in Rexed's laminae II (substantia gelatinosa). These distribution patterns indicated that SSEA-1 is present on migratory and/or postmigratory cells. In addition, SSEA-1 is associated with small-diameter dorsal root fibres, the C fibres and A(∂) fibres, that terminate within the substantia gelatinosa. After birth SSEA-1 was present throughout the dorsal horn, probably as a result of the myelination of the fibres. Chapman & Hall, 1992 Oudega Martin Neuroregulation Group, Department of Physiology, Leiden University, Wassenaarseweg 62, P.O. Box 9604, 2300 RC, Leiden, The Netherlands aut Marani Enrico Neuroregulation Group, Department of Physiology, Leiden University, Wassenaarseweg 62, P.O. Box 9604, 2300 RC, Leiden, The Netherlands aut Thomeer Rath Neuroregulation Group, Department of Neurosurgery, Leiden University, The Netherlands aut The Histochemical Journal Kluwer Academic Publishers 24/11(1992-11-01), 869-877 0018-2214 24:11<869 1992 24 10735 https://doi.org/10.1007/BF01046358 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046358 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oudega Martin Neuroregulation Group, Department of Physiology, Leiden University, Wassenaarseweg 62, P.O. Box 9604, 2300 RC, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Marani Enrico Neuroregulation Group, Department of Physiology, Leiden University, Wassenaarseweg 62, P.O. Box 9604, 2300 RC, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Thomeer Rath Neuroregulation Group, Department of Neurosurgery, Leiden University, The Netherlands aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/11(1992-11-01), 869-877 0018-2214 24:11<869 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer