caa a22 4500 469031093 CHVBK 20180323132743.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01046360 doi (NATIONALLICENCE)springer-10.1007/BF01046360 Expression of CD15 in tumours of the nervous system [Elektronische Daten] [Guido Reifenberger, Peter Sieth, Monika Niederhaus, Wolfgang Wechsler] Summary: The expression of the CD15 epitope was investigated by immunohistochemistry, western blotting and immuno-thin-layer chromatography on a large series of human nervous system tumours and ethylnitrosourea-induced rat gliomas. Our results show that CD15 is expressed as a glycoprotein- or glycolipid-associated epitope in normal human and rat brain. In contrast, immunoreactivity for CD15 was absent in tumour cells of experimental rat gliomas. In human tumours we found a more complex expression pattern. While intra- and perivascular granulocytes as well as macrophages in necrotic areas of anaplastic tumours were always strongly CD15-positive, immunoreactive tumour cells were detectable only in a fraction of low-grade gliomas. Anaplastic gliomas and glioblastomas consistently did not express the epitope on their tumour cells. In addition to individual low-grade gliomas, we found CD15-positive cases among metastatic carcinomas, craniopharyngeomas, meningiomas, germinomas and malignant melanomas. Our results suggest that immunohistochemistry for CD15 is potentially useful in diagnostic neuropathology as a marker for granulocytes in paraffin sections, as a supplementary tool for the histopathological grading of gliomas, and as an aid for differentiation between anaplastic glioma cells and non-neoplastic glia. Furthermore, it can be speculated that the lack of CD15 expression on anaplastic glioma cells may potentially be responsible for some of their characteristics-such as altered cellular interaction and loss of contact inhibition. Chapman & Hall, 1992 Reifenberger Guido Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut Sieth Peter Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut Niederhaus Monika Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut Wechsler Wolfgang Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut The Histochemical Journal Kluwer Academic Publishers 24/11(1992-11-01), 890-901 0018-2214 24:11<890 1992 24 10735 https://doi.org/10.1007/BF01046360 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046360 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reifenberger Guido Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut NATIONALLICENCE 700 1- Sieth Peter Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut NATIONALLICENCE 700 1- Niederhaus Monika Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut NATIONALLICENCE 700 1- Wechsler Wolfgang Department of Neuropathology, Heinrich-Heine-University, Moorenstraße 5, 4000, Düsseldorf 1, Germany aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/11(1992-11-01), 890-901 0018-2214 24:11<890 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer