caa a22 4500 469032405 CHVBK 20180323132747.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00216620 doi (NATIONALLICENCE)springer-10.1007/BF00216620 Impairement of MHC class I transcription in a mutant bovine B cell line [Elektronische Daten] [Jerome Harms, Gary Splitter] To better define the regulation and expression of bovine major histocompatibility complex (MHC) antigens, the bovine B lymphoblastoid cell line, BL3, was exposed to γ-irradiation and surviving cells were immunoselected for MHC class I antigen loss. The resulting class I expression loss variant, BL3.1, was characterized at both the protein and genetic levels to ascertain the nature of the defect. Microfluorimetry analysis revealed a 3-5-fold surface density reduction of all class I products on BL3.1 cells relative to the parental BL3 cells. This decreased surface expression was specific for MHC class I and not MHC class II or the non-MHC-linked gene product, immunoglobulin (Ig). Northern and quantitative slot blot analyses demostrated a corresponding diminution of class I RNA in BL3.1 suggesting a transcriptional level defect. Nuclear run-off and transcription inhibition experiments confirmed no post-transcriptional changes while Southern blot analysis provided no evidence for alterations within or near the class I genes. To help elucide the mechanism of altered class I expression, the parent, BL3, and variant, BL3.1, were cultured with factors known to enhance MHC class I transcription. Interferon (IFN)-γ, lipopolysaccharide (LPS), and activated peripheral cell lines induced MHC class I transcription and surface expression 2-3-fold greater than the untreated controls. It is likely, therefore, that a genetic alteration outside of the class I genes has occured within BL3.1 impairing expression of MHC class I. Springer-Verlag, 1992 Harms Jerome Department of Veterinary Science, University of Wisconsin-Madison, 1655 Linden Dr., 53706, Madison, WI, USA aut Splitter Gary Department of Veterinary Science, University of Wisconsin-Madison, 1655 Linden Dr., 53706, Madison, WI, USA aut Immunogenetics Springer-Verlag 35/1(1992-01-01), 1-8 0093-7711 35:1<1 1992 35 251 https://doi.org/10.1007/BF00216620 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00216620 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harms Jerome Department of Veterinary Science, University of Wisconsin-Madison, 1655 Linden Dr., 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Splitter Gary Department of Veterinary Science, University of Wisconsin-Madison, 1655 Linden Dr., 53706, Madison, WI, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/1(1992-01-01), 1-8 0093-7711 35:1<1 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer