caa a22 4500 469032472 CHVBK 20180323132747.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00216624 doi (NATIONALLICENCE)springer-10.1007/BF00216624 Mechanisms that generate junctional diversity in α and δ chains that use the Tcrd-V3 gene product [Elektronische Daten] [Philmore Holman, Michael Lacy, Matthew Roth, David Kranz] The signals that dictate whether a thymocyte will express the αß or γδ T-cell receptors are unknown. Although it is also not known if these two different cell types use identical recombinational machinery during rearrangement, the same variable (V) region genes can be used by both α and δ chains. By examining the products of rearrangements in αß or γδ thymocytes that express identical V genes, we hoped to determine whether these cell types might differ in particular aspects of their recombinational activity. The polymerase chain reaction was used to show that the Tcrd-V2, Tcrd-V3, and Tcra-V3 genes are expressed as both Tcra and Tcrd transcripts in fetal and adult BALB/c mice. Sequencing of Vδ3 isolates was performed in order to compare the contribution of various mechanisms to the generation of junctional diversity. Extensive junctional diversity was present at all stages of development examined (fetal, newborn, and adult). During early development both α and δ chain junctional diversity is generated primarily by variability in the position of joining two gene segments (i.e., Tcrd-V3 to Tcra-J in α chains; Tcrd-V3 to Tcrd-D2 and Tcrd-D2 to Tcrd-J1 in δ chains). The pattern of base pair deletion from the end of the Tcrd-V3 gene was identical in α and δ chains and deletions occurred in fetal as well as adult T cells. In later development T cells use not only this mechanism for α and δ chains but also the addition of bases at gene segment junctions, presumably through the action of terminal deoxynucleotidyl transferase (TdT). Finally, a comparison of the variable domains of these α and δ chains shows that a notable difference is the variability in length of the CDR 3 region which can be significantly longer in δ-chains than in α-chains. Springer-Verlag, 1992 Holman Philmore Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut Lacy Michael Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut Roth Matthew Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut Kranz David Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut Immunogenetics Springer-Verlag 35/1(1992-01-01), 33-40 0093-7711 35:1<33 1992 35 251 https://doi.org/10.1007/BF00216624 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00216624 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holman Philmore Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 700 1- Lacy Michael Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 700 1- Roth Matthew Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 700 1- Kranz David Department of Biochemistry, University of Illinois, 1209 West California Street, 61801, Urbana, Illinois, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/1(1992-01-01), 33-40 0093-7711 35:1<33 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer