caa a22 4500 469032723 CHVBK 20180323132747.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00185108 doi (NATIONALLICENCE)springer-10.1007/BF00185108 A Tcra congenic mouse: Vα epitope expression is influenced by both Tcra haplotypes and background genes [Elektronische Daten] [Lars Gleditsch, Bjarne Bogen] A T-cell receptor alpha chain locus (Tcra) congenic mouse is described. The Tcra a haplotype of BALB/c (donor strain) was bred on to B10.D2 (background strain, Tcra b haplotype) by using a Bgl I Tcra-C restriction fragment length polymorphism. Tcra a/b heterozygous offspring from the eleventh backross generation were brother-sister mated to obtain Tcra-C a homozygous animals. The resulting congenic line, B10.D2.C-Tcra a /Bo carries a recombination between the Tcra and the hr loci; thus, the transferred differential segment is the centromeric 18-27 cM of the BALB/c chromosome 14. Analysis with a multitude of Tcra-V and Tcrd-V probes demonstrates that the complete Tcra a haplotype is contained within this differential segment. Lymph node T cells of BALB/c (Tcra a ) B10.D2 (Tcra b ) and B10.D2.C-Tcra a were stained with anti-Vα8 (KT50, KT65), anti-Vα3.2 (RR3-16) and anti-Vα11.1 and 2 (RR8-1) monoclonal antibodies. We find that the frequencies of Vα epitope expression are highly Tcra haplotype-dependent even though an influence of background genes is also observed. Thus, Tcra-V germline differences may possibly influence the T cell repertoire, in addition to the already well known positive and negative thymic selections. Tcra haplotype does not influence the frequencies of Vβ utilization. However, BALB/c mice have fewer Vβ11+ T cells than B10.D2 and B10.D2-Tcra a , therefore, the BALB/c genome must harbor a Vβ11 deleting gene(s) in addition to those described so far. Springer-Verlag, 1992 Gleditsch Lars Institute of Immunology and Rheumatology, University of Oslo, Norway aut Bogen Bjarne Institute of Immunology and Rheumatology, University of Oslo, Norway aut Immunogenetics Springer-Verlag 35/3(1992-02-01), 153-160 0093-7711 35:3<153 1992 35 251 https://doi.org/10.1007/BF00185108 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185108 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gleditsch Lars Institute of Immunology and Rheumatology, University of Oslo, Norway aut NATIONALLICENCE 700 1- Bogen Bjarne Institute of Immunology and Rheumatology, University of Oslo, Norway aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/3(1992-02-01), 153-160 0093-7711 35:3<153 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer