caa a22 4500 469032731 CHVBK 20180323132748.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00185107 doi (NATIONALLICENCE)springer-10.1007/BF00185107 Transfection and expression of a gene coding for a human melanoma antigen recognized by autologous cytolytic T lymphocytes [Elektronische Daten] [Catia Traversari, Pierre van der Bruggen, Benoît Van den Eynde, Philippe Hainaut, Carine Lemoine, Nobuyoshi Ohta, Lloyd Old, Thierry Boon] Human melanoma line MZ2-MEL expresses several antigens recognized by autologous cytolytic T lymphocytes (CTL). As a first step towards the cloning of the gene coding for one of these antigens, we tried to obtain transfectants expressing the antigen. The DNA recipient cell was a variant of MZ2-MEL which had been selected with a CTL clone for the loss of antigen E. It was cotransfected with genomic DNA of the original melanoma line and with selective plasmid pSVtkneoβ. Geneticin-resistant transfectants were obtained at a frequency of 2 × 10−4. These transfectants were then screened for their ability to stimulate the production of tumor necrosis factor by the anti-E CTL clone. One transfectant expressing antigen E was identified among 70 000 drug-resistant transfectants. Its sensitivity to lysis by the anti-E CTL was equal to that of the original melanoma cell line. When this transfectant was submitted to immunoselection with the anti-E CTL clone, the resulting antigen-loss variants were found to have lost several of the transfected pSVtkneoβ sequences. This indicated that the gene coding for the antigen had been integrated in the vicinity of pSVtkneoβ sequences, as expected for cotransfected DNA. Springer-Verlag, 1992 Traversari Catia Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut van der Bruggen Pierre Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut Van den Eynde Benoît Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut Hainaut Philippe Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut Lemoine Carine Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut Ohta Nobuyoshi Ludwig Institute for Cancer Research, New York Unit at Memorial Sloan Kettering Cancer Center, 10025, New York, NY, USA aut Old Lloyd Ludwig Institute for Cancer Research, New York Unit at Memorial Sloan Kettering Cancer Center, 10025, New York, NY, USA aut Boon Thierry Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut Immunogenetics Springer-Verlag 35/3(1992-02-01), 145-152 0093-7711 35:3<145 1992 35 251 https://doi.org/10.1007/BF00185107 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185107 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Traversari Catia Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 700 1- van der Bruggen Pierre Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Van den Eynde Benoît Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Hainaut Philippe Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Lemoine Carine Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Ohta Nobuyoshi Ludwig Institute for Cancer Research, New York Unit at Memorial Sloan Kettering Cancer Center, 10025, New York, NY, USA aut NATIONALLICENCE 700 1- Old Lloyd Ludwig Institute for Cancer Research, New York Unit at Memorial Sloan Kettering Cancer Center, 10025, New York, NY, USA aut NATIONALLICENCE 700 1- Boon Thierry Ludwig Institute for Cancer Research, Brussels Branch, 74 avenue Hippocrate, B-1200, Brussels, Belgium aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/3(1992-02-01), 145-152 0093-7711 35:3<145 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer