caa a22 4500 469032758 CHVBK 20180323132748.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00185115 doi (NATIONALLICENCE)springer-10.1007/BF00185115 Gene duplications and sequence polymorphism of bovine class II DQB genes [Elektronische Daten] [Sunna Sigurdardóttir, Carina Borsch, Kenth Gustafsson, Leif Andersson] The genetic diversity of bovine class II DQB genes was investigated by polymerase chain reaction amplification and DNA sequencing. The first domain exon was amplified from genomic DNA samples representing 14 class II haplotypes, defined by restriction fragment length polymorphism (RFLP) analysis. The presence of a polymorphism in the copy number of DQB genes was confirmed since two DQB sequences were isolated from certain haplotypes. Four subtypes of bovine DQB genes were found. DQB1 is the major type and was found in almost all haplotypes. DQB2 is very similar DQB1 but was found only in the duplicated haplotypes DQ9 to 12. DQB3 and DQB4 are two quite divergent genes only present in certain duplicated haplotypes. The bovine DQB complexity thus resembles that in the human DRB region. Bovine DQB genes were found to be highly polymorphic as ten DQB1 alleles and four DQB2 alleles were identified. The observed sequence polymorphism correlated well with previously defined DQB RFLPs. Bovine and human DQB alleles show striking similarities at the amino acid level. In contrast, the frequency of silent substitutions is much higher in comparisons of DQB alleles between species than within species ruling out the possibility that any of the contemporary DQB alleles have been maintained since the divergence of humans and cattle. The frequency of silent substitutions between DQB alleles was markedly lower in cattle than in humans, in agreement with a previous comparison of human and bovine DRB alleles. Springer-Verlag, 1992 Sigurdardóttir Sunna Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Borsch Carina Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Gustafsson Kenth Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Andersson Leif Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Immunogenetics Springer-Verlag 35/3(1992-02-01), 205-213 0093-7711 35:3<205 1992 35 251 https://doi.org/10.1007/BF00185115 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185115 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sigurdardóttir Sunna Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Borsch Carina Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Gustafsson Kenth Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Andersson Leif Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/3(1992-02-01), 205-213 0093-7711 35:3<205 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469032758 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer