caa a22 4500 469032766 CHVBK 20180323132748.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00185113 doi (NATIONALLICENCE)springer-10.1007/BF00185113 Chromosome 14 in B10.A(18R) mice is recombinant and includes Tcra-V a alleles [Elektronische Daten] [Pamela Nakajima, John Di Vincenzo, Stephen Jameson, Nicholas Gascoigne] Analysis of mouse Tcr genes has previously defined at least five different Tcra-V haplotypes among inbred strains of mice. For mice of the Tcra-V b haplotype, including C57BL/10 (B10), T-cell expression of the Tcra-V11 gene subfamily can be detected with a monoclonal antibody, 1.F2. In the course of further characterizing the specificity of 1.F2, we found that it fails to recognize Tcra-V11-expressing T-cell hybrids derived from the B10 congenic strain, B10.A(18R)/SgIcr. Moreover, staining analysis indicated that the Va11 epitope recognized by 1.F2 is not expressed by peripheral T cells from several different B10.A(18R) colonies with the exception of that at the Research Institute of Scripps Clinic. Nucleotide sequences were determined for cDNA representing rearranged Tcra-V11 genes from two independent, B10.A(18R)/SgIcr derived T-cell hybrids. The two Tcra-V11 gene segments were identical and the predicted amino acid sequence differed by at least five residues from Tcra-V11 sequences previously obtained from B10.A mice. Southern blot analysis of restriction fragment length polymorphisms (RFLP) associated with Tcra-V11, as well as Tcra-V 1 , subfamily genes revealed that the B10.A(18R) mouse has inherited Tcra-V a alleles rather than the expected Tcra-V b alleles from the B10 strain. RFLP analysis of the Rib-1 locus, located in close proximity to the Tcra locus on chromosome 14, showed that B10.A(18R) carries the Rib-1 b allele from B10. These results indicate that the B10.A(18R) mouse has inherited a recombinant chromosome 14 with a recombination event having occured between the Rib-1 locus and the Tcra-V a gene subfamilies examined. Inheritance of Tcra-V a alleles in B10.A(18R) probably originated from strain 129/J which breeding records show was used in the first cross with B10.A in the production of B10.A(18R) and which we found exhibits Tcra-V11 a RFLPs. Springer-Verlag, 1992 Nakajima Pamela Department of Pathology, Fox Chase Cancer Center, 19111, Philadelphia, PA, USA aut Di Vincenzo John Department of Pathology, Fox Chase Cancer Center, 19111, Philadelphia, PA, USA aut Jameson Stephen Department of Immunology, Research Institute of Scripps Clinic, 92037, La Jolla, CA, USA aut Gascoigne Nicholas Department of Immunology, Research Institute of Scripps Clinic, 92037, La Jolla, CA, USA aut Immunogenetics Springer-Verlag 35/3(1992-02-01), 190-198 0093-7711 35:3<190 1992 35 251 https://doi.org/10.1007/BF00185113 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00185113 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakajima Pamela Department of Pathology, Fox Chase Cancer Center, 19111, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Di Vincenzo John Department of Pathology, Fox Chase Cancer Center, 19111, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Jameson Stephen Department of Immunology, Research Institute of Scripps Clinic, 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Gascoigne Nicholas Department of Immunology, Research Institute of Scripps Clinic, 92037, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/3(1992-02-01), 190-198 0093-7711 35:3<190 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer