caa a22 4500 469032979 CHVBK 20180323132748.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00189892 doi (NATIONALLICENCE)springer-10.1007/BF00189892 The multi-locus H-2D w16 region has an organization distinct from the D d region [Elektronische Daten] [Usha Tummuru, M. Zubairu, David Lee] Genomic DNA blot analyses using probes derived from the BALB/c 3′ flanking region of the L d gene (L d 3′ fl-C) and from near the BALB/c D3 d gene (50.2A) indicate that the B10.GAA37 mouse strain has a multi-locus D (D w16) region distinct from the five-gene organization observed in the D d and D q regions. To isolate the D w16 region class I genes, a genomic B10. GAA37-λEMBL3 library was generated and screened with probes that preferentially hybridize to K and D region class I genes. Hybridization analyses of the isolated λ clones with L d derived oligonucleotide probes suggested that one of the λ clones contained the L w16 gene, whereas several other λ clones contained the L w16 gene. The sequence of the D w16 gene is most similar to that of the D p gene, particularly in the 3′ half. Furthermore, the L w16 gene is quite similar in the 5′ half and virtually identical in the 3′ half to the L d gene, indicating that L w16, but not D w16, is a member of the L d gene family. Collectively, these data suggest that, through a D region recombination event, the novel D w16 region may have been assembled from primordial counterparts of the D p and L d genes. Springer-Verlag, 1992 Tummuru Usha Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut Zubairu M. Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut Lee David Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut Immunogenetics Springer-Verlag 35/5(1992-03-01), 296-308 0093-7711 35:5<296 1992 35 251 https://doi.org/10.1007/BF00189892 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00189892 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tummuru Usha Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Zubairu M. Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Lee David Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212, Columbia, MO, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 35/5(1992-03-01), 296-308 0093-7711 35:5<296 1992 35 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer