caa a22 4500 469033134 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00223539 doi (NATIONALLICENCE)springer-10.1007/BF00223539 Mhc-DRB diversity of the chimpanzee ( Pan troglodytes ) [Elektronische Daten] [M. Kenter, N. Otting, J. Anholts, M. Jonker, R. Schipper, R. Bontrop] Fifty-four chimpanzee Patr-DRB and five human HLA-DRB second exons were cloned and sequenced from thirty-five chimpanzees and four B-cell lines and compared with known Mhc-DRB sequences of these two species. Equivalents of the HLA-DRB1 * 02,-DRB1 * 03, -DRB1 * 07 allelic lineages and the HLA-DRB3,-DRB4, -DRB5, -DRB6, and -DRB7 loci were all found in the chimpanzee. In addition, two chimpanzee Patr-DRB lineages (Patr-DRBX and -DRBY) were found for which no human counterparts have been described. None of the Patr-DRB sequences is identical to known HLA-DRB sequences. The Patr-DRB1 * 0702 and HLA-DRB1 * 0701 alleles are the most similar sequences in a comparison between the two species and differ by only two nucleotides out of 246 sequences. Equivalents of the HLA-DRB1 * 01,-DRB1 * 04, and -DRB1 * 09 alleles were not found in our sample of chimpanzees. A per locus comparison of the number of Patr-DRB alleles with the HLA-DRB alleles shows that the Patr-DRB3, -DRB4, -DRB5, and -DRB6 locus are, thus far, more polymorphic than ther human homologs. The polymorphism of the Patr-DRB1 locus seems to be less extensive than that reported for the HLA-DRB1 locus. Nevertheless, the Patr-DRB1 locus seems to be the most polymorphic of the Patr-DRB loci. Phylogenetic analyses indicate that the HLA-DRB1 * 09 allele may have originated from a recombination between a Mhc-DRB5 allele and the DRB1 allele of a Mhc-DR7 haplotype. Although recombination seems to increase the diversity of the Patr-DRB alleles, its contribution to the generation of Patr-DRB variation is probably low. Hence, most Patr-DRB diversity presumably accumulated via recurrent point mutations. Finally, two distinct PAtr-DRB haplotypes are deduced, one of which (the chimpanzee equivalent of the HLA-Dr7 haplotype) is probably older than 6-8 million years. Springer-Verlag, 1992 Kenter M. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut Otting N. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut Anholts J. Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands aut Jonker M. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut Schipper R. Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands aut Bontrop R. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut Immunogenetics Springer-Verlag 37/1(1992-10-01), 1-11 0093-7711 37:1<1 1992 37 251 https://doi.org/10.1007/BF00223539 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00223539 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kenter M. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut NATIONALLICENCE 700 1- Otting N. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut NATIONALLICENCE 700 1- Anholts J. Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands aut NATIONALLICENCE 700 1- Jonker M. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut NATIONALLICENCE 700 1- Schipper R. Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands aut NATIONALLICENCE 700 1- Bontrop R. ITRI-TNO, P. O. Box 5815, 2280, HV Rijswijk, The Netherlands aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 37/1(1992-10-01), 1-11 0093-7711 37:1<1 1992 37 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer