caa a22 4500 469033169 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00223540 doi (NATIONALLICENCE)springer-10.1007/BF00223540 Trans-species origin of Mhc-DRB polymorphism in the chimpanzee [Elektronische Daten] [Werner Mayer, Colm O'hUigin, Zofia Zaleska-Rutczynska, Jan Klein] Trans-specific evolution of allelic polymorphism at the major histocompatibility complex loci has been demonstrated in a number of species. Estimating the substitution rates and the age of trans-specifically evolving alleles requires detailed information about the alleles in related species. We provide such information for the chimpanzee DRB genes. DNA fragments encompassing exon 2 were amplified in vitro from genomic DNA of ten chimpanzees. The nucleotide sequences were determined and their relationship to the human DRB alleles was evaluated. The alleles were classified according to their positioni in dendrograms and the presence of lineage-specific motifs. Twenty alleles were found at the expressed loci Patr-DRB1,-DRB3, -DRB4, -DRB5, and at the pseudogenes Patr-DRB6, -DRB7; of these, 13 are new alleles. Two other chimpanzee sequences were classified as members of a new lineage tentatively designated DRBX. Chimpanzee counterparts of HLA-DRB1 * 01 and * 04 were not detected. The number of alleles found at individual loci indicates asymmetrical distribution of polymorphism between humans and chimpanzees. Estimations of intra-lineage divergence times suggest that the lineages are more than 30 million year old. Predictions of major chimpanzee DRB haplotypes are made. Springer-Verlag, 1992 Mayer Werner Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut O'hUigin Colm Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut Zaleska-Rutczynska Zofia Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut Klein Jan Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut Immunogenetics Springer-Verlag 37/1(1992-10-01), 12-23 0093-7711 37:1<12 1992 37 251 https://doi.org/10.1007/BF00223540 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00223540 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mayer Werner Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- O'hUigin Colm Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Zaleska-Rutczynska Zofia Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Klein Jan Max-Planck-Institut für Biologie, Abteilung Immungenetik, W-7400, Tübingen, Germany aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 37/1(1992-10-01), 12-23 0093-7711 37:1<12 1992 37 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer