caa a22 4500 469033185 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00223544 doi (NATIONALLICENCE)springer-10.1007/BF00223544 Transmembrane and cytoplasmic domain sequences demonstrate at least two expressed bovine MHC class I loci [Elektronische Daten] [Shirley Ellis, Karen Braem, W. Morrison] We have used the polymerase chain reaction to amplify cDNA from expressed bovine major histocompatibility complex class I genes. Sequences obtained from transmembrane and cytoplasmic domains were used to identify the number of expressed alleles. Data from three animals suggest that there are four major expressed alleles, representing the products of two (or more) loci. We have also demonstrated the presence of an alternatively spliced mRNA, which has been observed in five animals. The alternative splicing removes exon 7 (the major site of class I phosphorylation), which predicts a truncated molecule with a cytoplasmic portion 16 amino acids shorter than usual. This phenomenon was detected for only a single class I allele within each individual. Springer-Verlag, 1992 Ellis Shirley Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut Braem Karen Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut Morrison W. Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut Immunogenetics Springer-Verlag 37/1(1992-10-01), 49-56 0093-7711 37:1<49 1992 37 251 https://doi.org/10.1007/BF00223544 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00223544 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ellis Shirley Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut NATIONALLICENCE 700 1- Braem Karen Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut NATIONALLICENCE 700 1- Morrison W. Institute for Animal Health, Nr Newbury, RG16 ONN, Compton, Berks, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 37/1(1992-10-01), 49-56 0093-7711 37:1<49 1992 37 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer