caa a22 4500 469033193 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00223543 doi (NATIONALLICENCE)springer-10.1007/BF00223543 Transcription and diversity of immunoglobulin lambda chain variable genes in the rat [Elektronische Daten] [Barbara Aguilar, George Gutman] In order to determine the extent of the repertoire of the immunoglobulin light chain V-region locus (Igl-V) in the laboratory rat (Rattus norvegicus), we constructed a specifically primed cDNA library from lipopolysaccharide-stimulated DA strain rat spleens. The library was screened with a rat Igl-C2-specific probe, and 33 clones containing identifiable V regions were sequenced, of which 19 sequences are presented here. In addition to one sequence (Igl-V1) which was already known, and is closely related to the two known mouse Vλ gene segments, clones encoding representatives of three new, distantly related, rat Igl-V subfamilies were found, namely Igl-V2, Igl-V3, and Igl-V4. At least two of these subfamilies. Igl-V2 and Igl-V3, contain multiple members as well as restriction fragment length polymorphism variants, indicating the presence of at least 10-15 Igl-V gene segments (including some pseudogenes) in the rat genome. An additional ten clones contained no rearranged V region, although they showed a correct J-C splice, suggesting the presence of cryptic transcriptional promoters between Jλ and the 3”-most Igl-V gene segment. Phylogenetic tree reconstruction based on amino acid sequence alignments showed at least three of the four rat Igl-V sequences clustering with distinct human Igl-V genes. Thus, although rats express λ-bearing Ig at levels no higher than mice, the rat Igl-V locus is considerably more complex than that of laboratory mice, and its diversity reflects the products of gene duplications which predate the time of primate/rodent divergence. Springer-Verlag, 1992 Aguilar Barbara Department of Microbiology and Molecular Genetics, University of California, 92717, Irvine, CA, USA aut Gutman George Department of Microbiology and Molecular Genetics, University of California, 92717, Irvine, CA, USA aut Immunogenetics Springer-Verlag 37/1(1992-10-01), 39-48 0093-7711 37:1<39 1992 37 251 https://doi.org/10.1007/BF00223543 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00223543 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aguilar Barbara Department of Microbiology and Molecular Genetics, University of California, 92717, Irvine, CA, USA aut NATIONALLICENCE 700 1- Gutman George Department of Microbiology and Molecular Genetics, University of California, 92717, Irvine, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 37/1(1992-10-01), 39-48 0093-7711 37:1<39 1992 37 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer