caa a22 4500 46903324X CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00209292 doi (NATIONALLICENCE)springer-10.1007/BF00209292 A possible association between basic amino acids of position 13 of DRB1 chains and autoimmune hepatitis [Elektronische Daten] [Masao Ota, Takeshi Seki, Kendo Kiyosawa, Seiichi Furuta, Kunihiko Hino, Toshiro Kondo, Hirofumi Fuskushima, Kimiyoshi Tsuji, Hidetoshi Inoko] Fifty-one patients with autoimmune hepatitis have been studied for HLA association by conventional serology and also by modified polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) genotyping.HLA-DR4 was significantly associated with autoimmmune hepatitis (46 of 51 patients, 90.2%). DNA typing of the DRB1 gene for 43 DR4-positive patients by using the PCR-RFLP technique revealed that of 43 patients, 33 had DRB1 * 0405 (Dw15), five had DRB1 * 0406 (DwKT2), four had DRB1 * 0403 (Dw13a), two had DRB1 * 0401 (Dw4), two of 43 had DRB1 * 0407 (Dw13b) and one had DRB1 * 0408 (Dw14b). Thus, there was no significant difference in Dw frequencies between DR4-positive patients and DR4-positive healthy subjects. These findings suggest that the DR4-specific sequence (Val 11 and His 13 at amino acid positions 11 and 13, respectively), but not particular Dw-associated DR4 sequence, in the first domain of the DRB1 chain contributes to susceptibility to autoimmune hepatitis among Japanese. Interestingly, all five of the DR4-negative patients had the DR2 specificity (DRB1 1502 or 1601). Taken together, these results imply that the basic amino acids at position 13, which is present only on the DR2 and DR4 B1 molecules (Arg on DR2 and His on DR4), are most important for determining the predisposition to autoimmune hepatitis. Springer-Verlag, 1992 Ota Masao Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut Seki Takeshi Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut Kiyosawa Kendo Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut Furuta Seiichi Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut Hino Kunihiko Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut Kondo Toshiro Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut Fuskushima Hirofumi Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut Tsuji Kimiyoshi Second Department of Transplantation, Tokai University School of Medicine, 259-11, Isehara, Japan aut Inoko Hidetoshi Second Department of Transplantation, Tokai University School of Medicine, 259-11, Isehara, Japan aut Immunogenetics Springer-Verlag 36/1(1992-05-01), 49-55 0093-7711 36:1<49 1992 36 251 https://doi.org/10.1007/BF00209292 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00209292 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ota Masao Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Seki Takeshi Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Kiyosawa Kendo Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Furuta Seiichi Second Department of Internal Medicine, Shinshu University School ofMedicine, asahi 3-1-1, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Hino Kunihiko Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut NATIONALLICENCE 700 1- Kondo Toshiro Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut NATIONALLICENCE 700 1- Fuskushima Hirofumi Second Department of Internal Medicine, National Defense Medical College, 359, Tokorozawa, Japan aut NATIONALLICENCE 700 1- Tsuji Kimiyoshi Second Department of Transplantation, Tokai University School of Medicine, 259-11, Isehara, Japan aut NATIONALLICENCE 700 1- Inoko Hidetoshi Second Department of Transplantation, Tokai University School of Medicine, 259-11, Isehara, Japan aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/1(1992-05-01), 49-55 0093-7711 36:1<49 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer