caa a22 4500 469033274 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00209287 doi (NATIONALLICENCE)springer-10.1007/BF00209287 Molecular characterization of the variable regions of a mouse polyreactive IgG2b antibody with rheumatoid factor activity [Elektronische Daten] [Anabelle Sequeira, Stratis Avrameas, Evelyne Jouvin-Marche] The complete nucleotide sequences of heavy and light chains of a mouse polyreactive IgG2b antibody were determined. This antibody, obtained after primary immunization of BALB/c mice with human lymphoblastoid cells, possess anti-HLA-DR and anti-rheumatoid factor activities and reacts with various self and nonself antigens. The VL and VH segments were found to belong to the VK8 and VH7183 families, respectively. The VH segment shared a high percentage of sequence similarity (95%) with previously described germline genes. The VK segment had 98.9% of sequence similarity with a consensus sequence VK8 of antibodies with anti-phosphorylcholine activity. Furthermore, the framework regions 2 and 3 of the VL segment were very similar to the framework regions 2 and 3 of other antibodies known to possess rheumatoid factor activity. We postulate that during immunization, the presence of HLA-DR antigens selects precursors having configurations similar to that of the germline, and induces some somatic mutations that do not significantly affect antibody polyreactivity. Springer-Verlag, 1992 Sequeira Anabelle Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France aut Avrameas Stratis Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France aut Jouvin-Marche Evelyne Unité d'Immunochimie Analytique, Institut Pasteur, Paris, France aut Immunogenetics Springer-Verlag 36/1(1992-05-01), 15-21 0093-7711 36:1<15 1992 36 251 https://doi.org/10.1007/BF00209287 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00209287 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sequeira Anabelle Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France aut NATIONALLICENCE 700 1- Avrameas Stratis Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France aut NATIONALLICENCE 700 1- Jouvin-Marche Evelyne Unité d'Immunochimie Analytique, Institut Pasteur, Paris, France aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/1(1992-05-01), 15-21 0093-7711 36:1<15 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer