caa a22 4500 469033320 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00218044 doi (NATIONALLICENCE)springer-10.1007/BF00218044 A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-γ in myeloid cells [Elektronische Daten] [Joseph Trapani, Kylie Browne, Michelle Dawson, Robert Ramsay, Roger Eddy, Thomas Shows, Perrin White, Bo Dupont] A cluster of at lest six interferon-γ (IFNγ)-inducible genes designated Ifi201-204 and located on mouse chromosome 1 has recently been described. Here , we report a human IFN-γ-inducible gene, IFI 16, which has nucleotide sequence similarity with portions of two of the mouse genes, Ifi202 and Ifi204. A full-length cDNA clone derived from IFI 16 [2.709 kilobases (kb)] contained a single open reading frame of 2.187 kb which encoded a putative polypeptide of 729 amino acids and a predicted non-glycosylated M r of 80020. IFI 16 mRNA was found to be constitutively expressed in lymphoid cells and in cell lines of both the T and B lineages. By contrast, the mRNA was not expresed by the cell lines HL-60, U937, and K562, which represent early stages of myeloid development, but was strongly inducible in HL-60 and U937 with IFN-γ. The IFI 16 protein demonstrated a putative domain structure with patchy similarity to the proteins expressed from gene Ifi202 and Ifi204. The mouse and human proteins each contain two analogous ≈200 amino acid domains which are imperfect copies, but IFI 16 demonstrated additional unique regions, including a Lys-rich N-terminal portion and a "spacer” region between the reiterated domains, analogous to spacer regions in the CD5 and CD8α molecules. Using a panel of inter-species somatic cell hybrid cell lines, IFI 16 was localized to the chromosomal region 1q12→1qter, a region systenic between mouse an man. DNA blotting indicated that, in contrast to the mouse, IFI 16 is present as a single copy gene in the human genome. The authors are pleased to make the cDNA clones described in this paper available to interested investigators. Springer-Verlag, 1992 Trapani Joseph The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut Browne Kylie The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut Dawson Michelle The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut Ramsay Robert Dewitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, NY, USA aut Eddy Roger Roswell Park Memorial Institute, 666 Elm Street, 14273, Buffalo, NY, USA aut Shows Thomas Roswell Park Memorial Institute, 666 Elm Street, 14273, Buffalo, NY, USA aut White Perrin Department of Pediatric Endocrinology, Cornell University Medical Center, 515 E71 Street, 10021, New York, NY, USA aut Dupont Bo Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, NY, USA aut Immunogenetics Springer-Verlag 36/6(1992-09-01), 369-376 0093-7711 36:6<369 1992 36 251 https://doi.org/10.1007/BF00218044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00218044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trapani Joseph The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut NATIONALLICENCE 700 1- Browne Kylie The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut NATIONALLICENCE 700 1- Dawson Michelle The Austin Research Institute, Austin Hospital, 3084, Heidelberg, Australia aut NATIONALLICENCE 700 1- Ramsay Robert Dewitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Eddy Roger Roswell Park Memorial Institute, 666 Elm Street, 14273, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Shows Thomas Roswell Park Memorial Institute, 666 Elm Street, 14273, Buffalo, NY, USA aut NATIONALLICENCE 700 1- White Perrin Department of Pediatric Endocrinology, Cornell University Medical Center, 515 E71 Street, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Dupont Bo Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, NY, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/6(1992-09-01), 369-376 0093-7711 36:6<369 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer