caa a22 4500 469033347 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00218043 doi (NATIONALLICENCE)springer-10.1007/BF00218043 Haplotypic origin of β-chain genes expressed by human T-cell clones [Elektronische Daten] [Eric Seboun, Narendra Joshi, Stephen Hauser] A contribution of allelic variation of T-cell receptor (Tcr) genes to the immune response has not been studied. Here we report that the presence of insertion-deletion-related polymorphisms (IDRP) of the Tcr β chain (Tcrb) can be utilized to distinguish the parental origin of the gene complex that undergoes rearrangement in individual T-cell clones. Phytohemagglutinin stimulated clones from an individual heterozygous for an IDRP located between the variable(V) and diversity (D)-joining (J) region genes were studied for the presence of V to DJ rearrangements in each of the two parental chromosomes. Results indicate that single rearrangements were present in the majority of clones. in contrast to the double rearrangements of D to J genes that were generally present. In this individual, V to DJ rearrangements also occured with different frequencies on each of the two germline genes. IDRP clonotyping of the Tcrb complex should prove generally applicable to the study of the influence of allelic variation of Tcrb genes in selection of the expressed T-cell repetoire. Springer-Verlag, 1992 Seboun Eric Neuroimmunology Unit, Massachusetts General Hospital, 02114, Boston, MA, USA aut Joshi Narendra Neuroimmunology Unit, Massachusetts General Hospital, 02114, Boston, MA, USA aut Hauser Stephen Department of Neurology, Massachusetts General Hospital, 32 Fruit Street, 02114, Boston, MA, USA aut Immunogenetics Springer-Verlag 36/6(1992-09-01), 363-368 0093-7711 36:6<363 1992 36 251 https://doi.org/10.1007/BF00218043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00218043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seboun Eric Neuroimmunology Unit, Massachusetts General Hospital, 02114, Boston, MA, USA aut NATIONALLICENCE 700 1- Joshi Narendra Neuroimmunology Unit, Massachusetts General Hospital, 02114, Boston, MA, USA aut NATIONALLICENCE 700 1- Hauser Stephen Department of Neurology, Massachusetts General Hospital, 32 Fruit Street, 02114, Boston, MA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/6(1992-09-01), 363-368 0093-7711 36:6<363 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer