caa a22 4500 469033363 CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00218045 doi (NATIONALLICENCE)springer-10.1007/BF00218045 Expression of mouse Tla region class I genes in tissue enriched for γδ cells [Elektronische Daten] [Pirooz Eghtesady, Kurt Brorson, Hilde Cheroutre, Robert Tigelaar, Leroy Hood, Mitchell Kronenberg] The Tla region of the BALB/c mouse major histocompatibility complex contains at least 20 class I genes. The function of the products of these genes is unknown, but recent evidence demonstrates that some Tla region gene products could be involved in presentation of antigens to γδ T cells. We have generated a set of polymerase chain reaction (PCR) oligonucleotide primers and hybridization probes that permit us to specifically amplify and detect expression of 11 of the 20 BALB/c Tla region genes. cDNA prepared from 12 adult and fetal tissues and from seven cell lines was analyzed. In some cases, northern blot analysis or staining with monoclonal antibodies specific for the Tla-encoded thymus leukemia (TL) antigen were used to confirm the expression pattern of several of the genes as determined by PCR. Some Tla region genes, such as T24 d and the members of the T10 d /T22 d gene pair, are expressed in a wide variety of tissues in a manner similar to the class I transplantation antigens. The members of the TL antigen encoding gene pair, T3 d /T18 d , are expressed in only a limited number of organs, including several sites enriched for γδ T cells. Other Tla region genes, including T1 d , T2 d , T16 d , and T17 d , are transcriptionally silent from the T8 d /T20 d gene pair do not undergo proper splicing. In general, sites that contain γδ T lymphocytes have Tla region transcripts. The newly identified pattern of expression of the genes analyzed in sites containing γδ T cells further extends the list of potential candidates for antigen presentation to γδ T cells. Springer-Verlag, 1992 Eghtesady Pirooz Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut Brorson Kurt Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, CA, USA aut Cheroutre Hilde Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut Tigelaar Robert Department of Dermatology and Internal Medicine, University of Texas Health Sciences Center, 75235, Dallas, TX, USA aut Hood Leroy Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, CA, USA aut Kronenberg Mitchell Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut Immunogenetics Springer-Verlag 36/6(1992-09-01), 377-388 0093-7711 36:6<377 1992 36 251 https://doi.org/10.1007/BF00218045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00218045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eghtesady Pirooz Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Brorson Kurt Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, CA, USA aut NATIONALLICENCE 700 1- Cheroutre Hilde Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Tigelaar Robert Department of Dermatology and Internal Medicine, University of Texas Health Sciences Center, 75235, Dallas, TX, USA aut NATIONALLICENCE 700 1- Hood Leroy Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, CA, USA aut NATIONALLICENCE 700 1- Kronenberg Mitchell Department of Microbiology and Immunology and the Jonsson Comprehensive Cancer Research Center, University of California, 90024, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/6(1992-09-01), 377-388 0093-7711 36:6<377 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469033363 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer