caa a22 4500 46903338X CHVBK 20180323132749.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00218042 doi (NATIONALLICENCE)springer-10.1007/BF00218042 Polymorphic analysis of the three MHC-linked HSP70 genes [Elektronische Daten] [Caroline Milner, R. Campbell] Three genes encoding members of the M r 70 000 heat shock protein family (HSP70) are known to lie in the class III region of the human major histocompatibility complex. IN order to determine whether these genes or their protein products exhibit any polymorphism the three genes have been specifically amplified from genomic DNA and sequenced. The HSP70-1 and HSP70-2 genes encode the major heat-inducible HSP70. A comparison of the nucleotide sequences of these genes from B8, SC01, DR3, B18, F1C30, DR3, and B7, SC30, DR2 haplotypes has revelad only very limited sequence variation which is not associated with any amino acid polymorphism. The HSP70-Hom gene encodes a protein that is highly related to HSP70-1, but which is not heat-inducible. Nucleotide sequence analysis of this gene from different haplotypes has revealed a Met → Thr amino acid substitution at residue 493 in a number of the haplotypes tested. This variable amino acid lies in the proposed peptide-binding site of the HSP70-Hom protein. Springer-Verlag, 1992 Milner Caroline Department of Biochemistry, MRC Immunochemistry Unit, South Parks, OX1 3QU, Oxford, UK aut Campbell R. Department of Biochemistry, MRC Immunochemistry Unit, South Parks, OX1 3QU, Oxford, UK aut Immunogenetics Springer-Verlag 36/6(1992-09-01), 357-362 0093-7711 36:6<357 1992 36 251 https://doi.org/10.1007/BF00218042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00218042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Milner Caroline Department of Biochemistry, MRC Immunochemistry Unit, South Parks, OX1 3QU, Oxford, UK aut NATIONALLICENCE 700 1- Campbell R. Department of Biochemistry, MRC Immunochemistry Unit, South Parks, OX1 3QU, Oxford, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/6(1992-09-01), 357-362 0093-7711 36:6<357 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer