caa a22 4500 469033452 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00661094 doi (NATIONALLICENCE)springer-10.1007/BF00661094 Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7 [Elektronische Daten] [Annamalai Selvakumar, Bhaskara Mohanraj, Roger Eddy, Thomas Shows, Perrin White, Bo Dupont] The human B lymphocyte activation antigen B7 provides regulatory signals for T lymphocytes as a consequence of binding to its ligands CD28 and CTLA-4. The cDNA for B7 has previously been isolated and predicted to encode a type I membrane protein. The predicted polypeptide has a secretory signal peptide followed by two contiguous Ig-like domains, a hydrophobic transmembrane region and a short cytoplasmic tail. Here we report the exon-intron genomic organization of human B7 and the chromosomal location. The gene has six exons that span approximately 32 kilobases of DNA. Exon 1 is not translated and the second exon contains the initiation ATG codon and encodes a predicted signal peptide. This gene structure is characteristic for several eukaryotic genes with tissue-specific expression. The third and fourth exons correspond to two Ig-like domains whereas the fifth and sixth exons encode respectively the trans-membrane portion and the cytoplasmic tail. This close relationship between exons and functional domains is a characteristic feature of genes of the Ig superfamily. Cell surface expression of the B7 gene product has previously been mapped to human chromosome 12 by antibody reactivity with the B7-specific monoclonal antibody BB-1. We here demonstrate that theB7 gene is located to theq21-qter region of chromosome 3 by DNA blot analysis of human × rodent somatic cell hybrids. Springer-Verlag, 1992 Selvakumar Annamalai Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut Mohanraj Bhaskara Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut Eddy Roger Department of Human Genetics, Rosewell Park Memorial Institute, 14263, Buffalo, NY, USA aut Shows Thomas Department of Human Genetics, Rosewell Park Memorial Institute, 14263, Buffalo, NY, USA aut White Perrin Division of Pediatric Endocrinology, Cornell University Medical College, 10021, New York, NY, USA aut Dupont Bo Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut Immunogenetics Springer-Verlag 36/3(1992-05-01), 175-181 0093-7711 36:3<175 1992 36 251 https://doi.org/10.1007/BF00661094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00661094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Selvakumar Annamalai Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Mohanraj Bhaskara Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Eddy Roger Department of Human Genetics, Rosewell Park Memorial Institute, 14263, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Shows Thomas Department of Human Genetics, Rosewell Park Memorial Institute, 14263, Buffalo, NY, USA aut NATIONALLICENCE 700 1- White Perrin Division of Pediatric Endocrinology, Cornell University Medical College, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Dupont Bo Human Immunogenetics Laboratory, Sloan-Kettering Institute for Cancer Research, 10021, New York, NY, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/3(1992-05-01), 175-181 0093-7711 36:3<175 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer