caa a22 4500 469033479 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00661095 doi (NATIONALLICENCE)springer-10.1007/BF00661095 Definition of microsatellite size variants for Tnfa and Hsp70 in autoimmune and nonautoimmune mouse strains [Elektronische Daten] [Chaim Jacob, Frances Hwang] We have cloned and sequenced the upstream regulatory region of tumor necrosis factor α (Tnfa) gene in 12 different mouse strains and identified an allelic polymorphism in the upstream regulatory region of the mouseTnfa gene. TheTNF allele found in the NZW strain is distinct from those of all otherH-2 haplotypes, supporting our previous suggestion that this allele may be associated with a regulatory or structural defect. In addition, simple tandem repeat sequences (microsatellites) within the promoter region of theTnfa gene and the 3′ untranslated region of one of the members of the HSP70 family (Hsp68c clone) were utilized as genetic markers. Ten TNF size variants and twelve HSP70 variants were identified in over forty mouse strains. Using these markers in a set of congenic mice, we mapped this member of the HSP70 family to the central portion of theH-2 complex, centromeric to theTnfa gene. The NOD and NZW strains carry uniqueHSP70 alleles based on the variability in the length of this marker. These findings raise the possibility that this protein may play a role in the association of the major histocompatibility complex with these autoimmune diseases. Springer-Verlag, 1992 Jacob Chaim Syntex Research, 94303, Palo Alto, CA, USA aut Hwang Frances Syntex Research, 94303, Palo Alto, CA, USA aut Immunogenetics Springer-Verlag 36/3(1992-05-01), 182-188 0093-7711 36:3<182 1992 36 251 https://doi.org/10.1007/BF00661095 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00661095 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jacob Chaim Syntex Research, 94303, Palo Alto, CA, USA aut NATIONALLICENCE 700 1- Hwang Frances Syntex Research, 94303, Palo Alto, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/3(1992-05-01), 182-188 0093-7711 36:3<182 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer