caa a22 4500 469033487 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00661091 doi (NATIONALLICENCE)springer-10.1007/BF00661091 Coordinate change in phenotype in a mouse cell line selected for CD8 expression [Elektronische Daten] [Robert Hyman, Valerie Stallings] A CD4+, CD8+ derivative of the CD4+, CD8− cell line SAKRTLS 12.1 was isolated by fluorescence activated cell sorting for CD8+ cells. This derivative showed a co-ordinate change in a number of independent characters: The parental cell line was CD4+, CD8−, CD3+, CD5hi, HSA+, DEXR, CD44hi, while the derivative was CD4+, CD8+, CD3−, CD510, HSA+, DEXS, CD4410. The derivative expressed the Thy-1.1, Ly-2.1, and Ly-3.1 surface antigens, consistent with origin from the SAKRTLS 12.1 parental cell line, and showed a drug resistance profile identical to that of the parent. It was not possible to isolate revertants with a phenotype identical to that of the parental cell line. Activation of the structural gene coding for CD8 α chain was correlated with demethylation at several sites. We interpret these results to mean that this CD8+ derivative of SAKRTLS 12.1 arose as a result of an alteration of a gene that coordinately regulates multiple genes whose expression changes during thymocyte differentiation. Gene methylation may contribute, directly or indirectly, to some or all of the changes in gene expression observed. Springer-Verlag, 1992 Hyman Robert Cancer Biology Laboratory, The Salk Institute, Box 85800, 92186-5800, San Diego, CA, USA aut Stallings Valerie Cancer Biology Laboratory, The Salk Institute, Box 85800, 92186-5800, San Diego, CA, USA aut Immunogenetics Springer-Verlag 36/3(1992-05-01), 149-156 0093-7711 36:3<149 1992 36 251 https://doi.org/10.1007/BF00661091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00661091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hyman Robert Cancer Biology Laboratory, The Salk Institute, Box 85800, 92186-5800, San Diego, CA, USA aut NATIONALLICENCE 700 1- Stallings Valerie Cancer Biology Laboratory, The Salk Institute, Box 85800, 92186-5800, San Diego, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/3(1992-05-01), 149-156 0093-7711 36:3<149 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer