caa a22 4500 469033525 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00215054 doi (NATIONALLICENCE)springer-10.1007/BF00215054 Interaction of H-2Eb with an IAP retrotransposon in the A20/2J B cell lymphoma [Elektronische Daten] [Carolyn Malstrom, Amy Kotsenas, Barry Jones] In the A20/2J BALB/c B cell lymphoma, Southern analysis revealed an insertion of approximately 6 kilobases of DNA into the first intron of one Eb d-allele. Two observations suggest that the rearrangement did not occur recently in the A20/2J subline. Firstly, normal and altered Eb d-alleles are present in equal numbers, and secondly, the LB 27.4 and LS 102.9 somatic cell hybrids formed at an earlier date both possess the rearrangement. Sequences of two cDNA clones, λEb-7 and λEb-125, selected from an A20/2J cDNA library prepared from poly [A+] RNA indicate that the rearranged Eb d-allele directs the synthesis of atypical Eb transcripts. The clones contain Eb sequence linked to a portion of retroviral-like intracisternal A-particle (IAP) genomic sequence, and they appear to be copies of mRNA produced by splicing between a 5′ donor site in the retroviral transcript and the 3′ acceptor site of the Eb gene's first intron. The longer λEb-125 insert corresponds to RNA that initiated in the 5′-untranslated region of the Eb gene. The 3′-end of the first Eb exon joins to long terminal repeat sequence, and retroviral sequence extends up to the splice junction with the second Eb exon; 3′ of the junction, the λEb-125 sequence corresponds to that of a correctly spliced Eb transcript. It seems feasible that the cDNA clones represent hybrid RNA synthesized by read-through transcription of the Eb coding region and an IAP element inserted into the first intron of the rearranged Eb d-allele. Springer-Verlag, 1992 Malstrom Carolyn Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut Kotsenas Amy Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut Jones Barry Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut Immunogenetics Springer-Verlag 36/4(1992-07-01), 238-247 0093-7711 36:4<238 1992 36 251 https://doi.org/10.1007/BF00215054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malstrom Carolyn Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Kotsenas Amy Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Jones Barry Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/4(1992-07-01), 238-247 0093-7711 36:4<238 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer