caa a22 4500 469033584 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00215049 doi (NATIONALLICENCE)springer-10.1007/BF00215049 Genomic organization of a polymorphic duplicated region centromeric of HLA-B [Elektronische Daten] [Chanvit Leelayuwat, Lawrence Abraham, Hyacinth Tabarias, Frank Christiansen, Roger Dawkins] The region between tumor necrosis factor (TNF) and HLA-B in the central major histocompatibility complex (MHC) is polymorphic and associated with several autoimmune diseases. The polymorphisms are haplospecific or haplotypic and retained within the same MHC ancestral haplotype (AH). We have cloned this region from four AHs into λ bacteriophage and found that a highly polymorphic region in the TNF-HLA-B interval is duplicated. Clones from this region isolated from three MHC AHs show two populations. The regions, designated CL1 and CL2, have different sizes of Bam HI fragments carrying the duplicated sequences. These fragments correspond to those seen after Bam HI restriction fragment length polymorphism (RFLP) analysis of genomic DNA from the same cell lines. Pulsed field gel electrophoresis analysis shows that both CL1 and CL2 are in the central MHC and are about 16 kilobases apart. DNA cloning and RFLP analysis demostrate that the Cl region is highly polymorphic but retained within an MHC AH. Polymorphism and duplication are common characteristics of the genes found in the MHC and therefore the CL sequences have the potential to be interesting in this respect. Springer-Verlag, 1992 Leelayuwat Chanvit Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut Abraham Lawrence Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut Tabarias Hyacinth Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut Christiansen Frank Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut Dawkins Roger Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut Immunogenetics Springer-Verlag 36/4(1992-07-01), 208-212 0093-7711 36:4<208 1992 36 251 https://doi.org/10.1007/BF00215049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leelayuwat Chanvit Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut NATIONALLICENCE 700 1- Abraham Lawrence Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut NATIONALLICENCE 700 1- Tabarias Hyacinth Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut NATIONALLICENCE 700 1- Christiansen Frank Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut NATIONALLICENCE 700 1- Dawkins Roger Department of Clinical Immunology, Royal Perth Hospital, Sir Charles Gairdner Hospital and the University of Western Australia, Perth, Western Australia aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/4(1992-07-01), 208-212 0093-7711 36:4<208 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer