caa a22 4500 469033657 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00215655 doi (NATIONALLICENCE)springer-10.1007/BF00215655 Molecular typing of HLA-B27 alleles [Elektronische Daten] [O. Dominguez, E. Coto, E. Martinez-Naves, S. Choo, C. López-Larrea] HLA-B27 represents a family of closely related antigens. Six alleles which differ in a limited number of nucleotide substitutions have been described (B*2701—B*2706). These changes are clustered in α1 and α2 domains. Polymerase chain reaction strategies were designed to amplify specific regions of class I exons 2 and 3. Amplified sequences were tested with eight sequence-specific oligonucleotides to distinguish all B27 subtypes. We also subtyped B27 in 50 healthy Spanish individuals using this procedure. The B*2705 subtype is over-represented in our population (96%). The remaining 4% carried the B*2702 allele. This finding is in agreement with the frequencies described by other techniques (cytotoxic T lymphocytes and isoeletric-focusing) for Caucasian populations. Class I oligotyping is a poorly developed field with significant potential applications. This procedure of genotyping B27 alleles is a reliable method which can be used in transplantation and B27-associated disease studies. Springer-Verlag, 1992 Dominguez O. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut Coto E. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut Martinez-Naves E. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut Choo S. Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut López-Larrea C. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut Immunogenetics Springer-Verlag 36/5(1992-08-01), 277-282 0093-7711 36:5<277 1992 36 251 https://doi.org/10.1007/BF00215655 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215655 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dominguez O. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Coto E. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Martinez-Naves E. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Choo S. Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut NATIONALLICENCE 700 1- López-Larrea C. Inmunologîa, Hospital Central, 33006, Oviedo, Spain aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/5(1992-08-01), 277-282 0093-7711 36:5<277 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer