caa a22 4500 469033673 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00215656 doi (NATIONALLICENCE)springer-10.1007/BF00215656 Inducible functions in hybrids of a Lyt-2+ BW5147 transfectant and the 2C CTL line [Elektronische Daten] [Jing Gu, Paul Gottlieb] Cytolytic activity and release of interleukin 2 (IL-2) were induced in Lyt-2-positive T-T cell hybrids by incubation with either concanavalin A or irradiated stimulator cells. Since hybrids of Lyt-2-positive class I-specific cytotoxic T lymphocytes (CTLs) with the fusable mouse thymoma cell line, BW5147, are invariably Lyt-2-negative, a derivative of BW5147 was produced by transfection which constitutively expresses surface Lyt-2.1. This cell line, 3B2, was fused with the H-2Ld-specific long term CTL line, 2C. Such hybrids expressed the transfected Lyt-2 gene but not the endogenous gene of the 2C fusion partner. That Lyt-2 plays a functional role in hybrids of 3b2 with 2C is shown by the observations that: 1) cytolysis by Lyt-2-positive hybrids was inhibited by Lyt-2-specific monoclonal antibody (mAb); 2) Lyt-2-positive but not Lyt-2-negative subclones of one such line develop specific cytotoxicity when incubated with stimulator cells; 3) Less IL-2 was released from Lyt-2-negative subclones incubated with stimulator cells than from Lyt-2-positive subclones; 4) Lyt-2-specific mAb inhibits release of IL-2 from Lyt-2-positive hybrids incubated with stimulator cells. All Lyt-2-positive hybrids expressed functional surface Lyt-3 encoded by the CTL fusion partner, demonstrating that expression of the Lyt-3 gene is not sensitive to the negative regulation which shuts off the endogenous Lyt-2 gene in hybrids of classI-specific CTLs with the 3B2 or BW5147 cell lines. The existence of inducible T-T cell hybrids expressing functional Lyt-2 and Lyt-3 provides a system for evaluation of the role(s) of Lyt-2 and Lyt-3 in the induction of function independent of cell growth. Springer-Verlag, 1992 Gu Jing Department of Microbiology, University of Texas at Austin, 78712, Austin, TX, USA aut Gottlieb Paul Department of Microbiology, University of Texas at Austin, 78712, Austin, TX, USA aut Immunogenetics Springer-Verlag 36/5(1992-08-01), 283-293 0093-7711 36:5<283 1992 36 251 https://doi.org/10.1007/BF00215656 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215656 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gu Jing Department of Microbiology, University of Texas at Austin, 78712, Austin, TX, USA aut NATIONALLICENCE 700 1- Gottlieb Paul Department of Microbiology, University of Texas at Austin, 78712, Austin, TX, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/5(1992-08-01), 283-293 0093-7711 36:5<283 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer