caa a22 4500 469033711 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00215659 doi (NATIONALLICENCE)springer-10.1007/BF00215659 Allelic polymorphism of mouse Igh-J locus, which encodes immunoglobulin heavy chain joining (JH) segments [Elektronische Daten] [Marja-Liisa Solin, Matti Kaartinen] The mouse genome contains four functional J H genes, which encode immunoglobulin heavy chain joining segments. The J H gene cluster is located a few kilobases 5′ from the constant region genes (C genes) on chromosome 12. The polymerase chain reaction (PCR)-technique was used to amplify DNA stretches from mouse genome of approximately 1 340 nucleotides in length containing all four J H genes (Igh-J locus). PCR products were directly used as templates in Sanger's dideoxy-sequencing, and sequences were determined. Twelve inbred mouse strains belonging to ten different Igh-C haplotypes were studied. The strains were: BALB/c, C58/J, RIII, DBA/2, CE, RF, CBA, NZB/J, AKR, C57BL/10, SJL, and A/J. Five allelic forms of the Igh-J locus were found among these strains. The A/J mouse has an allele (e) which differs from the BALB/c allele (a) by 15 nucleotides. C57BL and SJL have the allele (b) with eight differences from BALB/c. The CBA allele (j) has two differences, and the CE allele (f) has a single nucleotide difference compared with the BALB/c sequence. Based on the J H , variable (V) and constant (C) region sequences we conclude that independent reshuffling of V H ,J H , and C H gene clusters occurred during the evolution of Mus musculus. Springer-Verlag, 1992 Solin Marja-Liisa Deparment of Bacteriology and Immunology, University Helsinki, 00290, Helsinki, Finland aut Kaartinen Matti Deparment of Bacteriology and Immunology, University Helsinki, 00290, Helsinki, Finland aut Immunogenetics Springer-Verlag 36/5(1992-08-01), 306-313 0093-7711 36:5<306 1992 36 251 https://doi.org/10.1007/BF00215659 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215659 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Solin Marja-Liisa Deparment of Bacteriology and Immunology, University Helsinki, 00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Kaartinen Matti Deparment of Bacteriology and Immunology, University Helsinki, 00290, Helsinki, Finland aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/5(1992-08-01), 306-313 0093-7711 36:5<306 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer