caa a22 4500 46903372X CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00215660 doi (NATIONALLICENCE)springer-10.1007/BF00215660 Identification of novel human T-cell receptor Vβ gene segments by the anchored-polymerase chain reaction [Elektronische Daten] [Claudio Lunardi, Christopher Marguerie, Alex So] We have studied the diversity of the expressed human T-cell receptor (TCR) β-chain repertoire by analysis of mRNA from unstimulated peripheral blood T-cells. The anchored-polymerase chain reaction (PCR) was used to isolate TCRB transcripts. Of 20 full or near full-length functional transcripts sequenced, two were novel TCRVB gene segments. They have strong sequence similarities to the known TCRBV5, and 8 subfamilies. Southern blot analysis and sequence-specific oligonucleotide hybridization confirmed: a) that these sequences are present in genomic DNA; b) their relationship to the known TCRBV families. A TCRBV sequence similar to a recently identified novel TCRBV24 subfamily was also found. We show by southern blotting that this sequence forms a single member subfamily, and by deletion analysis of T-cell lines, we have mapped this sequence to lie between the genes which encode the TCRBV8.1 and TCRBV5.3 gene segments. The results show that the anchored PCR is a powerful tool in the analysis of the TCR repertoire, which may contain more V gene segments than previously defined. Springer-Verlag, 1992 Lunardi Claudio Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Marguerie Christopher Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut So Alex Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut Immunogenetics Springer-Verlag 36/5(1992-08-01), 314-318 0093-7711 36:5<314 1992 36 251 https://doi.org/10.1007/BF00215660 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215660 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lunardi Claudio Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Marguerie Christopher Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- So Alex Rheumatology Unit, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/5(1992-08-01), 314-318 0093-7711 36:5<314 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 439385040 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer