caa a22 4500 469033738 CHVBK 20180323132750.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00215661 doi (NATIONALLICENCE)springer-10.1007/BF00215661 Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene [Elektronische Daten] [Satoru Senju, Akinori Kimura, Michio Yasunami, Nobuhiro Kamikawaji, Hideyuki Yoshizumi, Yasuharu Nishimura, Takehiko Sasazuki] The β chain of the HLA-DQ molecule is shorter by eight amino acid residues than other major histocompatibility complex class II β chains due to elimination of the fifth exon coding for part of the cytoplasmic domain. This elimination is caused by one base substitution in the splice accepter site of the exon. We found that two HLA-DQB1 alleles, DQB1 * 0503 and DQB1 * 0601, did not have this substitution, and the exon was utilized in these two alleles. However, two forms of HLA-DQB mRNA, with or without exon 5, were generated in Epstein-Barr virus-transformed cell lines homozygous for DQB1 * 0503 or DQB1*0601, indicating alternative mRNA splicing. The alternative splicing of DQB1*0601 mRNA was also found in peripheral blood lymphocytes and L cell transfectants. To investigate the functional relevance of the allele-specific long cytoplasmic tail of HLA-DQ β chain, we developed three types of L cell transfectants expressing exclusively the HLA-DQw6 molecules with short cytoplasmic tail, long cytoplasmic tail, or both forms of the β chain, and used them as antigen presenting cells for streptococcal cell wall antigen-specific T cell lines. These three types of transfectants could function almost equally well as antigen presenting cells. It was thus demonstrated that both forms of HLA-DQ β chain, with or without eight amino acid residues coded for by the exon 5, can be associated with the HLA-DQ α chain, be expressed on the cell surface, and function as restriction molecules in antigen recognition by the CD4+ T cells. Springer-Verlag, 1992 Senju Satoru Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Kimura Akinori Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Yasunami Michio Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Kamikawaji Nobuhiro Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Yoshizumi Hideyuki Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Nishimura Yasuharu Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Sasazuki Takehiko Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut Immunogenetics Springer-Verlag 36/5(1992-08-01), 319-325 0093-7711 36:5<319 1992 36 251 https://doi.org/10.1007/BF00215661 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215661 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Senju Satoru Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kimura Akinori Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Yasunami Michio Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kamikawaji Nobuhiro Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Yoshizumi Hideyuki Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nishimura Yasuharu Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sasazuki Takehiko Department of Genetics, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/5(1992-08-01), 319-325 0093-7711 36:5<319 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer