caa a22 4500 469033770 CHVBK 20180323132751.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00215287 doi (NATIONALLICENCE)springer-10.1007/BF00215287 PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex [Elektronische Daten] [William Lafuse, Dennis Lanning, Thomas Spies, Chella David] We have constructed a long range restriction map of the S/D segment of the mouse H-2 complex by pulsed field gel electrophoresis and hybridization with mouse cDNA probes to Bf and Tnfa genes and human cDNA probes to BAT2, BAT3, BAT4, BAT5, and BAT6 genes which have recently been mapped to the human HLA complex between C2 and HLA-B. The distance between the mouse C2 and Tnfa genes was found to be approximately 350 kilobases. The position of the mouse Bat genes in this map were found to be comparable to the position of the BAT genes in the human HLA complex. A panel of recombinant mouse strains was also examined by restriction fragment analysis with probes detecting the Hsp70, Bat5, and Tnfa genes. The results indicate that recombination in this segment is not random. No recombinants were found with crossovers between the C2 and Hsp70 genes and only one recombinant was found with a crossover between Tnfa and H-2D. In contrast, the crossover sites of 16 recombinants were mapped between the Hsp70 and Tnfa genes. Seven of these recombinants were found to have crossovers between Hsp70 and Bat5 and three recombinants were found to have crossover sites between Bat5 and Tnfa. Springer-Verlag, 1992 Lafuse William Department of Medical Microbiology and Immunology, The Ohio State University, 333 W 10th Avenue, 43210, Columbus, OH, USA aut Lanning Dennis Department of Medical Microbiology and Immunology, The Ohio State University, 333 W 10th Avenue, 43210, Columbus, OH, USA aut Spies Thomas Division of Tumor Virology, Daan-Farber Cancer Institute, Harvard Medical School, 02115, Boston, MA, USA aut David Chella Department of Immunology, Mayo Clinic, 55905, Rochester, MN, USA aut Immunogenetics Springer-Verlag 36/2(1992-06-01), 110-116 0093-7711 36:2<110 1992 36 251 https://doi.org/10.1007/BF00215287 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00215287 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lafuse William Department of Medical Microbiology and Immunology, The Ohio State University, 333 W 10th Avenue, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Lanning Dennis Department of Medical Microbiology and Immunology, The Ohio State University, 333 W 10th Avenue, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Spies Thomas Division of Tumor Virology, Daan-Farber Cancer Institute, Harvard Medical School, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- David Chella Department of Immunology, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 36/2(1992-06-01), 110-116 0093-7711 36:2<110 1992 36 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer