caa a22 4500 469041994 CHVBK 20180323132813.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01998079 doi (NATIONALLICENCE)springer-10.1007/BF01998079 A greenhouse test for screening sugar-beet (Beta vulgaris) for resistance to beet necrotic yellow vein virus (BNYVV) [Elektronische Daten] [H. Paul, B. Henken, M. Alderlieste] Small differences in activity between batches of purified beet necrotic yellow vein virus (BNYVV) were observed in ELISA. A four-parameter modelled dose-response curve of purified BNYVV was used for the conversion of ELISA values to virus concentrations. Seedlings of the susceptible cultivar Regina and the partially resistant cultivars Nymphe and Rima were tested for resistance to BNYVV in a mixture of sand and infested soil. Plants were grown in a green-house with low nutrient supply and at temperatures below the optimum of both the vectorPolymyxa betae and BNYVV. Root systems were small and consisted mainly of lateral roots. Significant differences in average virus concentrations were found between cultivars, both when using the complete root systems and when using either the top or the bottom part of the root systems. Average virus concentrations in ‘Regina' were always significantly higher than in ‘Rima' and higher than in ‘Nymphe' on all occasions except one (P<0.05). Differences between ‘Nymphe' and ‘Rima' were less evident. Variation between plants was greatest within ‘Rima'. The test described in this paper can be used for the discrimination of different cultivars and for the identification of individual plants with resistance to BNYVV. Koninklijke Nederlandse Planteziektenkundige Vereniging, 1992 rhizomania nationallicence Polymyxa betae nationallicence ELISA nationallicence Paul H. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut Henken B. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut Alderlieste M. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/1(1992-01-01), 65-75 0028-2944 98:1<65 1992 98 10658 https://doi.org/10.1007/BF01998079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01998079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paul H. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut NATIONALLICENCE 700 1- Henken B. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut NATIONALLICENCE 700 1- Alderlieste M. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut NATIONALLICENCE 773 0- Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/1(1992-01-01), 65-75 0028-2944 98:1<65 1992 98 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer