caa a22 4500 469042281 CHVBK 20180323132814.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01974468 doi (NATIONALLICENCE)springer-10.1007/BF01974468 Torrance L. Virology Department, Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, UK aut Developments in methodology of plant virus detection [Elektronische Daten] [L. Torrance] As a general rule some form of polyclonal-antibody based enzyme immunoassay is still preferred for routine plant virus detection, but modifications may be necessary when increased sensitivity or specificity is required. In recent years new developments in antibody-based detection methods have mostly involved the provision of specific reagents, such as monoclonal antibodies or affinity-purified second antibody-enzyme conjugates which have helped to improve the sensitivity and specificity of the assays. Other kinds of tests (such as nucleic acid hybridisation) must be used when tests for virus coat protein are not appropriate. Recently, tests based on the polymerase chain reaction show great promise. There is no single universal test: assays must be devised and optimised for each pathogen. The challenge with all types of test is to make them quicker, less labour intensive, and —if possible — cheaper. Koninklijke Nederlandse Planteziektenkundige Vereniging, 1992 EIA nationallicence monoclonal antibody nationallicence potato nationallicence biotin nationallicence PCR nationallicence Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98(1992-03-01), 21-28 0028-2944 98<21 1992 98 10658 https://doi.org/10.1007/BF01974468 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974468 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Torrance L. Virology Department, Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, UK aut NATIONALLICENCE 773 0- Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98(1992-03-01), 21-28 0028-2944 98<21 1992 98 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer