caa a22 4500 469042540 CHVBK 20180323132815.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01996322 doi (NATIONALLICENCE)springer-10.1007/BF01996322 Franken A. Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut Application of polyclonal and monoclonal antibodies for the detection of Xanthomonas campestris pv. campestris in crucifer seeds using immunofluorescence microscopy [Elektronische Daten] [A. Franken] Polyclonal and monoclonal antibodies (PCAs and MCAs) were tested for the detection ofXanthomonas campestris pv.campestris (Xcc) in cabbage seeds using immunofluorescence microscopy (IF). It was concluded that PCA 94, MCAs 20H6, 2F4, 18G12 and a mixture of MCAs 20H6, 18G12, 2F4 and 16B5 could be used to detect Xcc in seed extracts when 5 min and 2.5 h shaking of seeds are used as extraction methods. The reliability of confirming suspect colonies with MCAs and PCA 94 in IF depended in part on the seed lot tested and the antibody used. Some virulent Xcc strains derived from seed lots, did not react with MCAs 10C5, 2F4, 18G12, 17C12 and 16B5. On the other hand, saprophytic isolates obtained from one seed lot cross-reacted with MCA 17C12 and to a lesser extent with MCAs 2F4, 18G12 and PCA 94. No relationship was found between IF-reactions of Xcc strains using MCAs and reactions of Xcc strains in pathogenicity testing. Xcc andX. c. pv.amoraciae (Xca) could in general not be distinguished on the basis of reactions with MCAs and PCAs. Also in pathogenicity tests Xcc and Xca were hard to distinguish. Koninklijke Nederlandse Planteziektenkundige Vereniging, 1992 pathogenicity testing nationallicence virulence nationallicence dilution-plating nationallicence YDC-agar nationallicence NSCA nationallicence NSCAA nationallicence nutrient starch medium nationallicence antibiotics nationallicence Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/2(1992-03-01), 95-106 0028-2944 98:2<95 1992 98 10658 https://doi.org/10.1007/BF01996322 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01996322 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Franken A. Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, the Netherlands aut NATIONALLICENCE 773 0- Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/2(1992-03-01), 95-106 0028-2944 98:2<95 1992 98 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer