caa a22 4500 469042583 CHVBK 20180323132815.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01996326 doi (NATIONALLICENCE)springer-10.1007/BF01996326 Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification, antiserum preparation, serological identification, and detection in pelargonium by ELISA [Elektronische Daten] [I. Bouwen, D. Maat] Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium. One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale ‘Springtime Irene'. The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from ‘Saturnus', collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed. PLPV could be reliably detected by ELISA inP. zonale ‘Springtime Irene' and ‘Amanda' throughout the year with only a few exceptions. InPelargonium peltatum ‘Tavira', however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested. Koninklijke Nederlandse Planteziektenkundige Vereniging, 1992 alfalfa mosaic virus nationallicence arabis mosaic virus nationallicence artichoke Italian latent virus nationallicence cucumber mosaic virus nationallicence indexing nationallicence pelargonium leaf curl virus nationallicence pelargonium ringspot virus nationallicence pelargonium vein clearing virus nationallicence pelargonium xonate spot virus nationallicence tobacco mosaic virus nationallicence tobacco necrosis virus nationallicence tobacco rattle virus nationallicence tobacco ringspot virus nationallicence tomato black ring virus nationallicence tomato ringspot virus nationallicence tomato spotted wilt virus nationallicence virus distribution nationallicence Bouwen I. DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW, Wageningen, the Netherlands aut Maat D. DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW, Wageningen, the Netherlands aut Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/2(1992-03-01), 141-156 0028-2944 98:2<141 1992 98 10658 https://doi.org/10.1007/BF01996326 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01996326 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bouwen I. DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW, Wageningen, the Netherlands aut NATIONALLICENCE 700 1- Maat D. DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW, Wageningen, the Netherlands aut NATIONALLICENCE 773 0- Netherlands Journal of Plant Pathology Kluwer Academic Publishers 98/2(1992-03-01), 141-156 0028-2944 98:2<141 1992 98 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer