caa a22 4500 469043822 CHVBK 20180323132818.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01800357 doi (NATIONALLICENCE)springer-10.1007/BF01800357 Leucine and tissue distribution of bulky and small neutral amino acids in rats: Dissociation between transport and insulin-mediated effects [Elektronische Daten] [C. de Céspedes, J. Thoene, K. Lowler, H. Christensen] Summary: The mechanism of the observed decrease in the plasma concentration of several amino acids in the presence of high levels of Leu has remained unexplained. In the present study a decrease in the plasma concentration of Ile, Val, Phe, Tyr, Met, Ala, Pro and Gly was observed after the intraperitoneal injection of Leu to weanling rats. Decreases in net intracellular concentrations in muscle accompanied the decrease in plasma of all of these amino acids except Pro and Gly. An increase in the distribution ratio muscle/plasma was observed exclusively for Gly after administration of Leu or of a non-insulinogenic transport system L analogue. Diazoxide suppressed the Leu-induced decreases in plasma and muscle intracellular concentrations of Ile and Val as well as of Pro in plasma. An increase in the distribution ratio liver/plasma was observed for Pro and Gly in the absence but not in the presence of diazoxide. All the above changes were statistically significant. Hence insulin probably mediates Leu effects, promoting an increased utilization of Ile and Val in muscle and of Pro in liver. A more direct effect of Leu appears to be involved in the apparent increased utilization of Phe, Tyr and Ala in the same tissue. Gly depletion in plasma can be explained by its trapping by inhibitory action of Leu on the exodus of Gly through transport system L. SSIEM and Kluwer Academic Publishers, 1992 de Céspedes C. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut Thoene J. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut Lowler K. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut Christensen H. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/1(1992-01-01), 145-154 0141-8955 15:1<145 1992 15 10545 https://doi.org/10.1007/BF01800357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01800357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Céspedes C. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut NATIONALLICENCE 700 1- Thoene J. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut NATIONALLICENCE 700 1- Lowler K. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut NATIONALLICENCE 700 1- Christensen H. Departments of Biological Chemistry and Pediatrics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA aut NATIONALLICENCE 773 0- Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/1(1992-01-01), 145-154 0141-8955 15:1<145 1992 15 10545 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer