caa a22 4500 469043881 CHVBK 20180323132818.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01800350 doi (NATIONALLICENCE)springer-10.1007/BF01800350 Effect of methylmalonate on in vitro lactate release and carbon dioxide production by brain of suckling rats [Elektronische Daten] [M. Wajner, J. Dutra, S. Cardoso, C. Wannmacher, E. Motta] Summary: Methylmalonate (MMA) accumulates in the tissues of patients with methylmalonic acidaemia, who present severe neurological signs soon after birth and later mental retardation. Attempting to understand the pathophysiology of the disorder, we investigated the effects of MMA on brain glucose uptake, lactate release and CO2 production. Glucose uptake and lactate release were studied by incubating 40 µm wide brain prisms from 15-day-old rats in Krebs-Ringer bicarbonate buffer, pH 7.0, containing 5.0 mmol/L glucose and one of three concentrations of MMA (1.0, 2.5 and 5.0 mmol/L). Controls did not contain MMA in the incubation medium. MMA induced a significant increase of lactate production in a dose-dependent pattern that was proportional to glucose uptake by the brain prisms. We also studied the influence of MMA on brain CO2 production from [2-14C]glucose and [U-14C]acetate by incubating brain prisms in the same buffer in the presence of the substrates with (experimental groups) or without (controls) 5.0 mmol/L MMA. MMA significantly reduced CO2 formation from both substrates. SSIEM and Kluwer Academic Publishers, 1992 Wajner M. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut Dutra J. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut Cardoso S. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut Wannmacher C. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut Motta E. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/1(1992-01-01), 92-96 0141-8955 15:1<92 1992 15 10545 https://doi.org/10.1007/BF01800350 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01800350 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wajner M. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Dutra J. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Cardoso S. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Wannmacher C. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Motta E. Departamento de Bioquimica, Instituto de Biociencias, UFRGS — Rua Sarmento Leite 500, 90.050, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/1(1992-01-01), 92-96 0141-8955 15:1<92 1992 15 10545 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer